Cloning of cell-specific secreted and surface proteins by subtractive antibody screening

Philipp E. Scherer, Perry E. Bickel, Mariana Kotler, Harvey F. Lodish

Research output: Contribution to journalArticlepeer-review

104 Scopus citations

Abstract

To identify and clone genes that encode cell- or tissue-specific secreted and surface proteins, a polyclonal antiserum was raised against a complex mixture of surface or secreted proteins from the target cell, followed by immunodepletion of antibodies that recognize proteins from a nontarget cell or tissue. The depleted antiserum is used to screen bacteriophage cDNA expression libraries. Because of our interest in how adipocytes communicate with other cells, we have used this method to clone cDNAs encoding secreted and plasma membrane proteins that are induced during adipocyte differentiation. We describe several of these, including a novel plasma membrane-associated protein, S3-12.

Original languageEnglish (US)
Pages (from-to)581-586
Number of pages6
JournalNature biotechnology
Volume16
Issue number6
DOIs
StatePublished - Jun 1998

Keywords

  • Adipocyte
  • Differential expression

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

Fingerprint

Dive into the research topics of 'Cloning of cell-specific secreted and surface proteins by subtractive antibody screening'. Together they form a unique fingerprint.

Cite this