Cloning the human and mouse MMS19 genes and functional complementation of a yeast mms19 deletion mutant

Lurdes Queimado, Malini Rao, Roger A. Schultz, Eugene V. Koonin, L. Aravind, Tiziana Nardo, Miria Stefanini, Errol C. Friedberg

Research output: Contribution to journalArticle

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Abstract

The MMS19 gene of the yeast Saccharomyces cerevisiae encodes a polypeptide of unknown function which is required for both nucleotide excision repair (NER) and RNA polymerase II (RNAP II) transcription. Here we report the molecular cloning of human and mouse orthologs of the yeast MMS19 gene. Both human and Drosophila MMS19 cDNAs correct thermosensitive growth and sensitivity to killing by UV radiation in a yeast mutant deleted for the MMS19 gene, indicating functional conservation between the yeast and mammalian gene products. Alignment of the translated sequences of MMS19 from multiple eukaryotes, including mouse and human, revealed the presence of several conserved regions, including a HEAT repeat domain near the C-terminus. The presence of HEAT repeats, coupled with functional complementation of yeast mutant phenotypes by the orthologous protein from higher eukaryotes, suggests a role of Mms19 protein in the assembly of a multiprotein complex(es) required for NER and RNAP II transcription. Both the mouse and human genes are ubiquitously expressed as multiple transcripts, some of which appear to derive from alternative splicing. The ratio of different transcripts varies in several different tissue types.

Original languageEnglish (US)
Pages (from-to)1884-1891
Number of pages8
JournalNucleic Acids Research
Volume29
Issue number9
StatePublished - May 1 2001

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Organism Cloning
Yeasts
RNA Polymerase II
Genes
Eukaryota
DNA Repair
Multiprotein Complexes
Sequence Alignment
Alternative Splicing
Molecular Cloning
Drosophila
Saccharomyces cerevisiae
Proteins
Complementary DNA
Radiation
Phenotype
Peptides
Growth

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Cite this

Queimado, L., Rao, M., Schultz, R. A., Koonin, E. V., Aravind, L., Nardo, T., ... Friedberg, E. C. (2001). Cloning the human and mouse MMS19 genes and functional complementation of a yeast mms19 deletion mutant. Nucleic Acids Research, 29(9), 1884-1891.

Cloning the human and mouse MMS19 genes and functional complementation of a yeast mms19 deletion mutant. / Queimado, Lurdes; Rao, Malini; Schultz, Roger A.; Koonin, Eugene V.; Aravind, L.; Nardo, Tiziana; Stefanini, Miria; Friedberg, Errol C.

In: Nucleic Acids Research, Vol. 29, No. 9, 01.05.2001, p. 1884-1891.

Research output: Contribution to journalArticle

Queimado, L, Rao, M, Schultz, RA, Koonin, EV, Aravind, L, Nardo, T, Stefanini, M & Friedberg, EC 2001, 'Cloning the human and mouse MMS19 genes and functional complementation of a yeast mms19 deletion mutant', Nucleic Acids Research, vol. 29, no. 9, pp. 1884-1891.
Queimado L, Rao M, Schultz RA, Koonin EV, Aravind L, Nardo T et al. Cloning the human and mouse MMS19 genes and functional complementation of a yeast mms19 deletion mutant. Nucleic Acids Research. 2001 May 1;29(9):1884-1891.
Queimado, Lurdes ; Rao, Malini ; Schultz, Roger A. ; Koonin, Eugene V. ; Aravind, L. ; Nardo, Tiziana ; Stefanini, Miria ; Friedberg, Errol C. / Cloning the human and mouse MMS19 genes and functional complementation of a yeast mms19 deletion mutant. In: Nucleic Acids Research. 2001 ; Vol. 29, No. 9. pp. 1884-1891.
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