Cognate DNA binding specificity retained after leucine zipper exchange between GCN4 and C/EBP

Peter Agre, Peter F. Johnson, Steven L. McKnight

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

Both C/EBP and GCN4 are sequence-specific DNA binding proteins that control gene expression. Recent evidence implicates C/EBP as a transcriptional regulator of genes involved in lipid and carbohydrate metabolism. The C/EBP protein binds avidly to the dyad symmetric sequence 5′-ATTGCGCAAT- 3′; GCN4 regulates the transcription of genes that control amino acid biosynthesis in yeast, and binds avidly to the dyad symmetric sequence 5′-ATGA(G/C)TCAT-3′. Both C/EBP and GCN4 bind DNA via the same structural motif. This motif has been predicted to be bipartite, consisting of a dimerization interface termed the "leucine zipper" and a DNA contact surface termed the "basic region." Specificity of DNA binding has been predicted to be imparted by the basic region. As a test of this hypothesis, recombinant proteins were created wherein the basic regions and leucine zippers of GCN4 and C/EBP were reciprocally exchanged. In both of the recombinant polypeptides, DNA binding specificity is shown to track with the basic region.

Original languageEnglish (US)
Pages (from-to)922-926
Number of pages5
JournalScience
Volume246
Issue number4932
StatePublished - 1989

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Leucine Zippers
DNA
CCAAT-Enhancer-Binding Proteins
Recombinant DNA
Carbohydrate Metabolism
DNA-Binding Proteins
Dimerization
Regulator Genes
Lipid Metabolism
Recombinant Proteins
Yeasts
Gene Expression
Amino Acids
Peptides
Genes

ASJC Scopus subject areas

  • General

Cite this

Cognate DNA binding specificity retained after leucine zipper exchange between GCN4 and C/EBP. / Agre, Peter; Johnson, Peter F.; McKnight, Steven L.

In: Science, Vol. 246, No. 4932, 1989, p. 922-926.

Research output: Contribution to journalArticle

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