Colocalization of calcium-dependent protease II and one of its substrates at sites of cell adhesion

Mary C. Beckerle, Keith Burridge, George N. DeMartino, Dorothy E. Croall

Research output: Contribution to journalArticle

207 Citations (Scopus)

Abstract

Adhesion plaques, specialized regions of the plasma membrane where a cell contacts its substratum, are dynamic structures. However, little is known about how the protein-protein interactions that occur at adhesion plaques are controlled. One mechanism by which a cell might modulate its associations with the substratum is by selective, regulated proteolysis of an adhesion plaque component. Here we show that the catalytic subunit of the calcium-dependent protease type II (CDP-II) is localized in adhesion plaques of several cell types (BS-C-1, EBTr, and MDBK). We have compared the susceptibility of the adhesion plaque constituents vinculin, talin, and α-actinin to calcium-dependent proteolysis in vitro and have found talin to be the preferred substrate for CDP-II. The colocalization of a calcium-requiring proteolytic enzyme and talin in adhesion plaques raises the possibility that calcium-dependent proteolytic activity provides a mechanism for regulating some aspect of adhesion plaque physiology and function via cleavage of talin.

Original languageEnglish (US)
Pages (from-to)569-577
Number of pages9
JournalCell
Volume51
Issue number4
DOIs
StatePublished - Nov 20 1987

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Focal Adhesions
Cell adhesion
Cell Adhesion
Talin
Adhesion
Calcium
Substrates
Proteolysis
Vinculin
Actinin
Physiology
Cell membranes
Staphylococcus aureus auR protein
Catalytic Domain
Proteins
Peptide Hydrolases
Cell Membrane

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Colocalization of calcium-dependent protease II and one of its substrates at sites of cell adhesion. / Beckerle, Mary C.; Burridge, Keith; DeMartino, George N.; Croall, Dorothy E.

In: Cell, Vol. 51, No. 4, 20.11.1987, p. 569-577.

Research output: Contribution to journalArticle

Beckerle, Mary C. ; Burridge, Keith ; DeMartino, George N. ; Croall, Dorothy E. / Colocalization of calcium-dependent protease II and one of its substrates at sites of cell adhesion. In: Cell. 1987 ; Vol. 51, No. 4. pp. 569-577.
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