Colocalization of calcium-dependent protease II and one of its substrates at sites of cell adhesion

Mary C. Beckerle, Keith Burridge, George N. DeMartino, Dorothy E. Croall

Research output: Contribution to journalArticlepeer-review

212 Scopus citations


Adhesion plaques, specialized regions of the plasma membrane where a cell contacts its substratum, are dynamic structures. However, little is known about how the protein-protein interactions that occur at adhesion plaques are controlled. One mechanism by which a cell might modulate its associations with the substratum is by selective, regulated proteolysis of an adhesion plaque component. Here we show that the catalytic subunit of the calcium-dependent protease type II (CDP-II) is localized in adhesion plaques of several cell types (BS-C-1, EBTr, and MDBK). We have compared the susceptibility of the adhesion plaque constituents vinculin, talin, and α-actinin to calcium-dependent proteolysis in vitro and have found talin to be the preferred substrate for CDP-II. The colocalization of a calcium-requiring proteolytic enzyme and talin in adhesion plaques raises the possibility that calcium-dependent proteolytic activity provides a mechanism for regulating some aspect of adhesion plaque physiology and function via cleavage of talin.

Original languageEnglish (US)
Pages (from-to)569-577
Number of pages9
Issue number4
StatePublished - Nov 20 1987

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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