Combined effect of fluoride and arsenate on gene expression of osteoclast differentiation factor and osteoprotegerin

Lin Jia, Tai Yi Jin

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10 Scopus citations

Abstract

Objective: To study the combined effect of fluoride and arsenate on the expression of SD rat osteoblastic osteoclast differentiation factor (ODF) mRNA and osteoprotegerin (OPG) mRNA. Methods: Osteoblasts were obtained by enzymatic isolation from newborn SD rats. A factorial experiment was performed. Osteoblasts were exposed to NaF (0.5 mmolF/L, 4 molF/L) and NB 3AsH2 (12.5 μmolAs/L and 200 μmolAs/L) separately or F plus As and cultured for 48 h. The gene expression of osteoblastic ODF and OPG was observed by RT-PCR. Results: The expression of ODF mRNA increased in F0.5, F4 groups compared with control group and two groups of F0.5As200, F4As200 compared with As200 group, and decreased significantly in groups of F 4As125, F0.5As200, and F 4As200. The expression of OPG mRNA decreased in groups of F4, As200, F4As12.5, F 0.5As200, and F4As200. Conclusion: The joint effect of fluoride and arsenate on the gene expression of ODF is antagonistic, while the combined effect on the gene expression of OPG is synergistic. F4, F4As12.5, and F 0.5As200 promote bone resorption of rat osteoclasts, whereas F0.5As12.5 inhibits osteolytic effect of rat osteoclasts.

Original languageEnglish (US)
Pages (from-to)375-379
Number of pages5
JournalBiomedical and Environmental Sciences
Volume19
Issue number5
Publication statusPublished - Oct 1 2006

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Keywords

  • Arsenate
  • Combined effect
  • Fluoride
  • ODF
  • OPG

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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