Compact molten globule-like state of hUBF HMG Box1 at extremely low pH

Xuecheng Zhang, Jiahai Zhang, Xuan Li, Junjie Xu, Hongda Huang, Quan Chen, Jihui Wu, Yunyu Shi

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Using far and near-UV CD, ANS fluorescence and 2D NMR spectroscopy, an acid-induced partly folded state (A state) at extremely low pH for hUBF HMG Box1 was identified and characterized. As compared to the native state (N), the A state has similar secondary structure, less compact pack with larger amounts of exposed hydrophobic surface, and narrower chemical shift dispersion in 1H-15N HSQC spectrum, which implies that it is a molten globule (MG)-like species. On the other hand, substantial tertiary contacts and cooperative thermal denaturing transition indicate that the A state is closer-relative to the classic MG-to the native folded state. In addition, when the solution pH is adjusted to neutrality, the protein in the A state refolds to the native state easily. All these data suggest that the A state of hUBF HMG Box1 could represent a potential folding intermediate on protein folding pathway.

Original languageEnglish (US)
Pages (from-to)66-73
Number of pages8
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1748
Issue number1
DOIs
StatePublished - Apr 15 2005

Keywords

  • Acid-induced state
  • Molten globule
  • Protein folding
  • hUBF HMG Box1

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biophysics
  • Biochemistry
  • Molecular Biology

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