Comparative analysis of microarray data identifies common responses to caloric restriction among mouse tissues

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60 Scopus citations

Abstract

Caloric restriction has been extensively investigated as an intervention that both extends lifespan and delays age-related disease in mammals. In mice, much interest has centered on evaluating gene expression changes induced by caloric restriction (CR) in particular tissue types, but the overall systemic effect of CR among multiple tissues has been examined less extensively. This study presents a comparative analysis of microarray datasets that have collectively examined the effects of CR in 10 different tissue types (liver, heart, muscle, hypothalamus, hippocampus, white adipose tissue, colon, kidney, lung and cochlea). Using novel methods for comparative analysis of microarray data, detailed comparisons of the effects of CR among tissues are provided, and 28 genes for which expression response to CR is most shared among tissues are identified. These genes characterize common responses to CR, which consist of both activation and inhibition of stress-response pathways. With respect to liver tissue, transcriptional effects of CR exhibited surprisingly little overlap with those of aging, and a variable degree of overlap with the potential CR-mimetic drug resveratrol. These analyses shed light on the systemic transcriptional activity associated with CR diets, and also illustrate new approaches for comparative analysis of microarray datasets in the context of aging biology.

Original languageEnglish (US)
Pages (from-to)138-153
Number of pages16
JournalMechanisms of Ageing and Development
Volume129
Issue number3
DOIs
StatePublished - Mar 2008
Externally publishedYes

Keywords

  • Aging
  • Diet restriction
  • Gene expression
  • Longevity
  • Microarray

ASJC Scopus subject areas

  • Aging
  • Developmental Biology

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