Comparative immunoproteomics of idenfication and characterization of virulence factors from helicobacter pylori related to gastric cancer

Yu Fen Lin, Ming Shiang Wu, Chia Che Chang, Sheng Wei Lin, Jaw Town Lin, Yuh Ju Sun, Ding Shinn Chen, Lu Ping Chow

Research output: Contribution to journalArticle

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Abstract

Helicobacter pylori is an important risk factor of gastric cancer (GC). Although many H. pylori virulence factors have been reported, the pathogenic mechanism by which H. pylori infection causes GC remains unclear. The aims of this study were to identify GC-related antigens from H. pylori and characterize their roles in the development of GC. As GC and duodenal ulcer (DU) are considered clinically divergent, we compared two-dimensional immunoblots of an acid-glycine extract of H. pylori probed with serum samples from 15 patients with GC and 15 with DU to find GC-related antigens, which were subsequently identified by mass spectrometry. Many protein spots were recognized by more than one serum, and 24 of these were better recognized by GC sera. The proteins showing higher frequency of recognition in GC group are threonine synthase, rod shape-determining protein, S-adenosylmethionine synthetase, peptide chain release factor 1, DNA-directed RNA polymerase α subunit, co-chaperonin GroES (monomeric and dimeric forms), response regulator OmpR, and membrane fusion protein. Of these proteins, GroES was identified as a dominant GC-related antigen with a much higher seropositivity of GC samples (64.2%, n = 95) compared with 30.9% for gastritis (n = 94) and 35.5% for DU (n = 124). GroES seropositivity was more commonly associated with antral GC than with non-antral GC (odds ratio = 2.7; 95% confidence interval, 1.1-6.7). In peripheral blood mononuclear cells, GroES stimulated production of interleukin (IL)-8, IL-6, granulocyte macrophage colony-stimulating factor, IL-1β, tumor necrosis factor-α, cyclooxygenase-2, and prostaglandin E2. Moreover when incubated with gastric epithelial cells, GroES induced expression of IL-8, cell proliferation, and up-regulation of c-jun, c-fos, and cyclin D1 but caused down-regulation of p27Kip1. We conclude that GroES of H. pylori is a novel GC-associated virulence factor and may contribute to gastric carcinogenesis via induction of inflammation and promotion of cell proliferation.

Original languageEnglish (US)
Pages (from-to)1484-1496
Number of pages13
JournalMolecular and Cellular Proteomics
Volume5
Issue number8
DOIs
StatePublished - Aug 2006

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Cell proliferation
Virulence Factors
Interleukin-8
Helicobacter pylori
Stomach Neoplasms
Chaperonin 10
Membrane Fusion Proteins
Methionine Adenosyltransferase
Chaperonins
Antigens
Protein S
Cyclin D1
Cyclooxygenase 2
DNA-Directed RNA Polymerases
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-1
Dinoprostone
Glycine
Mass spectrometry
Interleukin-6

ASJC Scopus subject areas

  • Biochemistry

Cite this

Comparative immunoproteomics of idenfication and characterization of virulence factors from helicobacter pylori related to gastric cancer. / Lin, Yu Fen; Wu, Ming Shiang; Chang, Chia Che; Lin, Sheng Wei; Lin, Jaw Town; Sun, Yuh Ju; Chen, Ding Shinn; Chow, Lu Ping.

In: Molecular and Cellular Proteomics, Vol. 5, No. 8, 08.2006, p. 1484-1496.

Research output: Contribution to journalArticle

Lin, Yu Fen ; Wu, Ming Shiang ; Chang, Chia Che ; Lin, Sheng Wei ; Lin, Jaw Town ; Sun, Yuh Ju ; Chen, Ding Shinn ; Chow, Lu Ping. / Comparative immunoproteomics of idenfication and characterization of virulence factors from helicobacter pylori related to gastric cancer. In: Molecular and Cellular Proteomics. 2006 ; Vol. 5, No. 8. pp. 1484-1496.
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abstract = "Helicobacter pylori is an important risk factor of gastric cancer (GC). Although many H. pylori virulence factors have been reported, the pathogenic mechanism by which H. pylori infection causes GC remains unclear. The aims of this study were to identify GC-related antigens from H. pylori and characterize their roles in the development of GC. As GC and duodenal ulcer (DU) are considered clinically divergent, we compared two-dimensional immunoblots of an acid-glycine extract of H. pylori probed with serum samples from 15 patients with GC and 15 with DU to find GC-related antigens, which were subsequently identified by mass spectrometry. Many protein spots were recognized by more than one serum, and 24 of these were better recognized by GC sera. The proteins showing higher frequency of recognition in GC group are threonine synthase, rod shape-determining protein, S-adenosylmethionine synthetase, peptide chain release factor 1, DNA-directed RNA polymerase α subunit, co-chaperonin GroES (monomeric and dimeric forms), response regulator OmpR, and membrane fusion protein. Of these proteins, GroES was identified as a dominant GC-related antigen with a much higher seropositivity of GC samples (64.2{\%}, n = 95) compared with 30.9{\%} for gastritis (n = 94) and 35.5{\%} for DU (n = 124). GroES seropositivity was more commonly associated with antral GC than with non-antral GC (odds ratio = 2.7; 95{\%} confidence interval, 1.1-6.7). In peripheral blood mononuclear cells, GroES stimulated production of interleukin (IL)-8, IL-6, granulocyte macrophage colony-stimulating factor, IL-1β, tumor necrosis factor-α, cyclooxygenase-2, and prostaglandin E2. Moreover when incubated with gastric epithelial cells, GroES induced expression of IL-8, cell proliferation, and up-regulation of c-jun, c-fos, and cyclin D1 but caused down-regulation of p27Kip1. We conclude that GroES of H. pylori is a novel GC-associated virulence factor and may contribute to gastric carcinogenesis via induction of inflammation and promotion of cell proliferation.",
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AU - Chow, Lu Ping

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N2 - Helicobacter pylori is an important risk factor of gastric cancer (GC). Although many H. pylori virulence factors have been reported, the pathogenic mechanism by which H. pylori infection causes GC remains unclear. The aims of this study were to identify GC-related antigens from H. pylori and characterize their roles in the development of GC. As GC and duodenal ulcer (DU) are considered clinically divergent, we compared two-dimensional immunoblots of an acid-glycine extract of H. pylori probed with serum samples from 15 patients with GC and 15 with DU to find GC-related antigens, which were subsequently identified by mass spectrometry. Many protein spots were recognized by more than one serum, and 24 of these were better recognized by GC sera. The proteins showing higher frequency of recognition in GC group are threonine synthase, rod shape-determining protein, S-adenosylmethionine synthetase, peptide chain release factor 1, DNA-directed RNA polymerase α subunit, co-chaperonin GroES (monomeric and dimeric forms), response regulator OmpR, and membrane fusion protein. Of these proteins, GroES was identified as a dominant GC-related antigen with a much higher seropositivity of GC samples (64.2%, n = 95) compared with 30.9% for gastritis (n = 94) and 35.5% for DU (n = 124). GroES seropositivity was more commonly associated with antral GC than with non-antral GC (odds ratio = 2.7; 95% confidence interval, 1.1-6.7). In peripheral blood mononuclear cells, GroES stimulated production of interleukin (IL)-8, IL-6, granulocyte macrophage colony-stimulating factor, IL-1β, tumor necrosis factor-α, cyclooxygenase-2, and prostaglandin E2. Moreover when incubated with gastric epithelial cells, GroES induced expression of IL-8, cell proliferation, and up-regulation of c-jun, c-fos, and cyclin D1 but caused down-regulation of p27Kip1. We conclude that GroES of H. pylori is a novel GC-associated virulence factor and may contribute to gastric carcinogenesis via induction of inflammation and promotion of cell proliferation.

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