Comparison of spontaneous and induced mutation rates in an immortalized human bronchial epithelial cell line and its tumorigenic derivative

Jay L. Wittenkeller, Barry Storer, Gerard Bittner, Joan H. Schiller

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

To determine the relationship between neoplastic transformation and increased genetic instability, spontaneous and induced mutation rates were compared in a nontumorigenic, immortalized human bronchial epithelial cell line (NL20) and a tumorigenic cell line (NL20T) spontaneously derived from the NL20 line. Using the hypoxanthine phosphoribosyltransferase (HPRT) locus as a marker for determining mutation rate, fluctuation analysis was utilized to evaluate the spontaneous mutation rate. Induced mutation rates were determined for each cell line after N-methyl-N’-nitro-N-nitrosoguanidine exposure. Both the spontaneous and induced mutation rates were noted to be significantly higher in the nontumorigenic NL20 cell line. These findings suggest that increasing genetic instability, as measured by spontaneous or induced mutation rate in the HPRT locus, does not correlate with tumorigenicity in these cells.

Original languageEnglish (US)
Pages (from-to)335-341
Number of pages7
JournalOncology (Switzerland)
Volume54
Issue number4
DOIs
StatePublished - Jan 1 1997

Keywords

  • Bronchial epithelial cells, human
  • Fluctuation analysis
  • Hypoxanthine phosphoribosyltransferase
  • Transformation Mutation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Comparison of spontaneous and induced mutation rates in an immortalized human bronchial epithelial cell line and its tumorigenic derivative'. Together they form a unique fingerprint.

  • Cite this