TY - JOUR
T1 - Concordance in biomarker status between bladder tumors at time of transurethral resection and subsequent radical cystectomy
T2 - Results of a 5-year prospective study
AU - Passoni, Niccolò M.
AU - Shariat, Shahrokh F.
AU - Bagrodia, Aditya
AU - Francis, Franto
AU - Rachakonda, Varun
AU - Xylinas, Evanguelos
AU - Kapur, Payal
AU - Sagalowsky, Arthur I
AU - Lotan, Yair
N1 - Publisher Copyright:
© 2016 - IOS Press and the authors. All rights reserved.
PY - 2016
Y1 - 2016
N2 - Purpose: To assess the concordance rate in alterations of molecular markers at the time of transurethral resection (TUR) and subsequent radical cystectomy (RC) among patients with high-grade urothelial carcinoma of the bladder (UCB). Methods: We prospectively performed immunohistochemical staining p53, p21, p27, Ki-67 and cyclin E1 on TUR and on RC specimens from 102 patients treated with RC and bilateral lymphadenectomy for high-grade UCB.We analyzed the concordance rate of individual markers and of the number of altered markers. Concordant and discordant findings were reported in the overall population and according to clinical stage. Results: Median patient age was 74 years (IQR 67-79) and mostly male (86%). Median time from TUR to RC was 1.5 months (IQR 1.0-2.4). Clinical stage at time of RC was cTa/Tis/T1 in 50%, cT2 in 47%, and cT4 in 1% of patients Nine (9%) patients received neoadjuvant chemotherapy. The concordance of biomarkers between TUR and RC specimens was 92.2%, 77.5%, 80.4%, 77.5%, and 83.3% for cyclin E1, p21, p27, p53 and Ki-67, respectively. The concordance between number of altered biomarkers was 51.0%. Conclusions: The rate of individual marker alterations at time of TUR closely approximates that found at RC specimens. However, the correlation of number of altered markers is lower. Molecular marker status at TUR could help predict the marker status at RC and may help guide multimodal therapeutic planning.
AB - Purpose: To assess the concordance rate in alterations of molecular markers at the time of transurethral resection (TUR) and subsequent radical cystectomy (RC) among patients with high-grade urothelial carcinoma of the bladder (UCB). Methods: We prospectively performed immunohistochemical staining p53, p21, p27, Ki-67 and cyclin E1 on TUR and on RC specimens from 102 patients treated with RC and bilateral lymphadenectomy for high-grade UCB.We analyzed the concordance rate of individual markers and of the number of altered markers. Concordant and discordant findings were reported in the overall population and according to clinical stage. Results: Median patient age was 74 years (IQR 67-79) and mostly male (86%). Median time from TUR to RC was 1.5 months (IQR 1.0-2.4). Clinical stage at time of RC was cTa/Tis/T1 in 50%, cT2 in 47%, and cT4 in 1% of patients Nine (9%) patients received neoadjuvant chemotherapy. The concordance of biomarkers between TUR and RC specimens was 92.2%, 77.5%, 80.4%, 77.5%, and 83.3% for cyclin E1, p21, p27, p53 and Ki-67, respectively. The concordance between number of altered biomarkers was 51.0%. Conclusions: The rate of individual marker alterations at time of TUR closely approximates that found at RC specimens. However, the correlation of number of altered markers is lower. Molecular marker status at TUR could help predict the marker status at RC and may help guide multimodal therapeutic planning.
KW - Bladder cancer
KW - Cystectomy
KW - Molecular markers
KW - Transurethral resection of bladder tumors
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U2 - 10.3233/BLC-150036
DO - 10.3233/BLC-150036
M3 - Article
C2 - 27376130
AN - SCOPUS:85002704838
SN - 2352-3727
VL - 2
SP - 91
EP - 99
JO - Bladder Cancer
JF - Bladder Cancer
IS - 1
ER -