TY - JOUR
T1 - Consecutive radiofluorography and silver staining of two‐dimensional gel electrophoretograms
T2 - Application in determining the biosynthesis of serum and tissue proteins
AU - Kuhn, L.
AU - Kettman, J.
AU - Lefkovits, I.
PY - 1989
Y1 - 1989
N2 - The methodology for conventional radiofluorography of two‐dimensional gels, followed by rehydration of the gel and subsequent silver staining, is described. The image obtained by radiofluorography is referred to as biosynthetic image, and the image obtained by silver staining as constitutive image. Since the two images are already in close register (the same gel), reliable identification of polypeptides by the two different assays is possible, and the comparison provides valuable information on the catabolism of each entity. The utility of this procedure is illustrated in experiments involving a labeling with L‐[35S] methionine of an entire mouse. Both serum and tissue samples were analyzed by two‐dimensional gel electrophoresis with the aim of determining several categories of polypeptides in terms of their biosynthetic rates and their composition.
AB - The methodology for conventional radiofluorography of two‐dimensional gels, followed by rehydration of the gel and subsequent silver staining, is described. The image obtained by radiofluorography is referred to as biosynthetic image, and the image obtained by silver staining as constitutive image. Since the two images are already in close register (the same gel), reliable identification of polypeptides by the two different assays is possible, and the comparison provides valuable information on the catabolism of each entity. The utility of this procedure is illustrated in experiments involving a labeling with L‐[35S] methionine of an entire mouse. Both serum and tissue samples were analyzed by two‐dimensional gel electrophoresis with the aim of determining several categories of polypeptides in terms of their biosynthetic rates and their composition.
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U2 - 10.1002/elps.1150101009
DO - 10.1002/elps.1150101009
M3 - Article
C2 - 2482173
AN - SCOPUS:0024414853
SN - 0173-0835
VL - 10
SP - 708
EP - 713
JO - ELECTROPHORESIS
JF - ELECTROPHORESIS
IS - 10
ER -