Construction of a genomic map of Moraxella (Branhamella) catarrhalis ATCC 25238 and physical mapping of virulence-associated genes

K. T. Nguyen, E. J. Hansen, M. A. Farinha

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A physical genome map of the Moraxella catarrhalis type strain (ATCC 25238) has been constructed using pulsed field gel electrophoresis. Macrorestriction analyses of the genome of M. catarrhalis were performed by digestion with the restriction enzymes Sinai, NotI, and RsrII, which cleave the single circular chromosome into 9, 10, and 6 fragments, respectively. The chromosomal fragments generated by pulsed field gel electrophoresis were convened to a linkage map utilizing a combination of partial digestions, and cross-hybridizations. Moraxella catarrhalis, like a number of other respiratory pathogens, has a relatively small genome estimated at 1750 kilobase pairs or about 40% of the size of the Escherichia coli genome. The locations of the four ribosomal RNA operons (rrnLS) were determined by Southern hybridization and by digestion with I-CeuI endonuclease. A number of genes involved in virulence have been placed onto the physical map by Southern hybridization including those encoding the predominant outer- membrane proteins and the chromosomal gene encoding β-lactamase.

Original languageEnglish (US)
Pages (from-to)299-303
Number of pages5
JournalCanadian Journal of Microbiology
Volume45
Issue number4
DOIs
StatePublished - 1999

Fingerprint

Moraxella (Branhamella) catarrhalis
Virulence
Genes
Genome
Digestion
Pulsed Field Gel Electrophoresis
Electrophoresis
Chromosomes, Human, Pair 9
Gels
Ribosomal RNA
Operon
Gene encoding
Pathogens
Chromosomes
Membrane Proteins
Escherichia coli
Enzymes

Keywords

  • Genome analysis
  • Moraxella catarrhalis
  • Physical map
  • Pulsed-field gel electrophoresis
  • Virulence

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Immunology
  • Microbiology

Cite this

Construction of a genomic map of Moraxella (Branhamella) catarrhalis ATCC 25238 and physical mapping of virulence-associated genes. / Nguyen, K. T.; Hansen, E. J.; Farinha, M. A.

In: Canadian Journal of Microbiology, Vol. 45, No. 4, 1999, p. 299-303.

Research output: Contribution to journalArticle

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