Control of occlusion of middle cerebral artery in perinatal and neonatal mice with magnetic force

Jie Min Jia, Chuanqi Peng, Yihui Wang, Jie Zheng, Woo Ping Ge

Research output: Contribution to journalArticle

Abstract

Ischemic perinatal stroke (IPS) is common, resulting in significant mortality and morbidity. In such cases, the incidence of unilateral arterial cerebral infarction is often occluded in the middle cerebral artery (MCA), leading to focal ischemia. In adult rodents, blockage of MCA is the most frequently used strategy for ischemic stroke study. However, modeling MCA occlusion (MCAo) in postnatal day 0-7 (P0-7) mouse pups for IPS study has not been accomplished. Here we occluded the dMCA by inducing the accumulation of magnetic particles (MPs) administered through the superficial temporal vein of mice between P0 and P7, which we called neonatal or perinatal SIMPLE (Stroke Induced with Magnetic Particles). SIMPLE produced either permanent or transient occlusion in the dMCA of perinatal and neonatal mice. Permanent MCA occlusion with SIMPLE resulted in cerebral infarction and neuronal death in the brain. SIMPLE can also be used to reliably produce focal ischemic stroke in neonatal or perinatal mouse brains. As a result, SIMPLE allows the modeling of IPS or focal ischemic stroke for further mechanistic studies in mice, with particular utility for mimicking transient focal ischemia in human pre-term babies, which for the first time here has been accomplished in mice.

Original languageEnglish (US)
Article number47
JournalMolecular Brain
Volume11
Issue number1
DOIs
StatePublished - Aug 29 2018

Fingerprint

Middle Cerebral Artery Infarction
Stroke
Cerebral Infarction
Middle Cerebral Artery
Ischemia
Brain Death
Veins
Rodentia
Morbidity

Keywords

  • Distal MCA
  • Ischemic neonatal stroke
  • Ischemic perinatal stroke
  • Magnetic force
  • Magnetic nanoparticles
  • SIMPLE

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Control of occlusion of middle cerebral artery in perinatal and neonatal mice with magnetic force. / Jia, Jie Min; Peng, Chuanqi; Wang, Yihui; Zheng, Jie; Ge, Woo Ping.

In: Molecular Brain, Vol. 11, No. 1, 47, 29.08.2018.

Research output: Contribution to journalArticle

Jia, Jie Min ; Peng, Chuanqi ; Wang, Yihui ; Zheng, Jie ; Ge, Woo Ping. / Control of occlusion of middle cerebral artery in perinatal and neonatal mice with magnetic force. In: Molecular Brain. 2018 ; Vol. 11, No. 1.
@article{fb0cdd1dadaa44a5a6e0e1e30ac5dd64,
title = "Control of occlusion of middle cerebral artery in perinatal and neonatal mice with magnetic force",
abstract = "Ischemic perinatal stroke (IPS) is common, resulting in significant mortality and morbidity. In such cases, the incidence of unilateral arterial cerebral infarction is often occluded in the middle cerebral artery (MCA), leading to focal ischemia. In adult rodents, blockage of MCA is the most frequently used strategy for ischemic stroke study. However, modeling MCA occlusion (MCAo) in postnatal day 0-7 (P0-7) mouse pups for IPS study has not been accomplished. Here we occluded the dMCA by inducing the accumulation of magnetic particles (MPs) administered through the superficial temporal vein of mice between P0 and P7, which we called neonatal or perinatal SIMPLE (Stroke Induced with Magnetic Particles). SIMPLE produced either permanent or transient occlusion in the dMCA of perinatal and neonatal mice. Permanent MCA occlusion with SIMPLE resulted in cerebral infarction and neuronal death in the brain. SIMPLE can also be used to reliably produce focal ischemic stroke in neonatal or perinatal mouse brains. As a result, SIMPLE allows the modeling of IPS or focal ischemic stroke for further mechanistic studies in mice, with particular utility for mimicking transient focal ischemia in human pre-term babies, which for the first time here has been accomplished in mice.",
keywords = "Distal MCA, Ischemic neonatal stroke, Ischemic perinatal stroke, Magnetic force, Magnetic nanoparticles, SIMPLE",
author = "Jia, {Jie Min} and Chuanqi Peng and Yihui Wang and Jie Zheng and Ge, {Woo Ping}",
year = "2018",
month = "8",
day = "29",
doi = "10.1186/s13041-018-0389-0",
language = "English (US)",
volume = "11",
journal = "Molecular Brain",
issn = "1756-6606",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Control of occlusion of middle cerebral artery in perinatal and neonatal mice with magnetic force

AU - Jia, Jie Min

AU - Peng, Chuanqi

AU - Wang, Yihui

AU - Zheng, Jie

AU - Ge, Woo Ping

PY - 2018/8/29

Y1 - 2018/8/29

N2 - Ischemic perinatal stroke (IPS) is common, resulting in significant mortality and morbidity. In such cases, the incidence of unilateral arterial cerebral infarction is often occluded in the middle cerebral artery (MCA), leading to focal ischemia. In adult rodents, blockage of MCA is the most frequently used strategy for ischemic stroke study. However, modeling MCA occlusion (MCAo) in postnatal day 0-7 (P0-7) mouse pups for IPS study has not been accomplished. Here we occluded the dMCA by inducing the accumulation of magnetic particles (MPs) administered through the superficial temporal vein of mice between P0 and P7, which we called neonatal or perinatal SIMPLE (Stroke Induced with Magnetic Particles). SIMPLE produced either permanent or transient occlusion in the dMCA of perinatal and neonatal mice. Permanent MCA occlusion with SIMPLE resulted in cerebral infarction and neuronal death in the brain. SIMPLE can also be used to reliably produce focal ischemic stroke in neonatal or perinatal mouse brains. As a result, SIMPLE allows the modeling of IPS or focal ischemic stroke for further mechanistic studies in mice, with particular utility for mimicking transient focal ischemia in human pre-term babies, which for the first time here has been accomplished in mice.

AB - Ischemic perinatal stroke (IPS) is common, resulting in significant mortality and morbidity. In such cases, the incidence of unilateral arterial cerebral infarction is often occluded in the middle cerebral artery (MCA), leading to focal ischemia. In adult rodents, blockage of MCA is the most frequently used strategy for ischemic stroke study. However, modeling MCA occlusion (MCAo) in postnatal day 0-7 (P0-7) mouse pups for IPS study has not been accomplished. Here we occluded the dMCA by inducing the accumulation of magnetic particles (MPs) administered through the superficial temporal vein of mice between P0 and P7, which we called neonatal or perinatal SIMPLE (Stroke Induced with Magnetic Particles). SIMPLE produced either permanent or transient occlusion in the dMCA of perinatal and neonatal mice. Permanent MCA occlusion with SIMPLE resulted in cerebral infarction and neuronal death in the brain. SIMPLE can also be used to reliably produce focal ischemic stroke in neonatal or perinatal mouse brains. As a result, SIMPLE allows the modeling of IPS or focal ischemic stroke for further mechanistic studies in mice, with particular utility for mimicking transient focal ischemia in human pre-term babies, which for the first time here has been accomplished in mice.

KW - Distal MCA

KW - Ischemic neonatal stroke

KW - Ischemic perinatal stroke

KW - Magnetic force

KW - Magnetic nanoparticles

KW - SIMPLE

UR - http://www.scopus.com/inward/record.url?scp=85052755112&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85052755112&partnerID=8YFLogxK

U2 - 10.1186/s13041-018-0389-0

DO - 10.1186/s13041-018-0389-0

M3 - Article

C2 - 30157965

AN - SCOPUS:85052755112

VL - 11

JO - Molecular Brain

JF - Molecular Brain

SN - 1756-6606

IS - 1

M1 - 47

ER -