Convergent evolution in the BAHD family of acyl transferases: Identification and characterization of anthocyanin acyl transferases from Arabidopsis thaliana

Jie Luo, Yasutaka Nishiyama, Christine Fuell, Goro Taguchi, Katherine Elliott, Lionel Hill, Yoshikazu Tanaka, Masahiko Kitayama, Mami Yamazaki, Paul Bailey, Adrian Parr, Anthony J. Michael, Kazuki Saito, Cathie Martin

Research output: Contribution to journalArticle

128 Scopus citations

Abstract

Members of the BAHD family of plant acyl transferases are very versatile catalytically, and are thought to be able to evolve new substrate specificities rapidly. Acylation of anthocyanins occurs in many plant species and affects anthocyanin stability and light absorption in solution. The versatility of BAHD acyl transferases makes it difficult to identify genes encoding enzymes with defined substrate specificities on the basis of structural homology to genes of known catalytic function alone. Consequently, we have used a modification to standard functional genomics strategies, incorporating co-expression profiling with anthocyanin accumulation, to identify genes encoding three anthocyanin acyl transferases from Arabidopsis thaliana. We show that the activities of these enzymes influence the stability of anthocyanins at neutral pH, and some acylations also affect the anthocyanin absorption maxima. These properties make the BAHD acyl transferases suitable tools for engineering anthocyanins for an improved range of biotechnological applications.

Original languageEnglish (US)
Pages (from-to)678-695
Number of pages18
JournalPlant Journal
Volume50
Issue number4
DOIs
StatePublished - May 1 2007

Keywords

  • Acyl transferases
  • Anthocyanins
  • Convergent evolution
  • Functional genomics

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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    Luo, J., Nishiyama, Y., Fuell, C., Taguchi, G., Elliott, K., Hill, L., Tanaka, Y., Kitayama, M., Yamazaki, M., Bailey, P., Parr, A., Michael, A. J., Saito, K., & Martin, C. (2007). Convergent evolution in the BAHD family of acyl transferases: Identification and characterization of anthocyanin acyl transferases from Arabidopsis thaliana. Plant Journal, 50(4), 678-695. https://doi.org/10.1111/j.1365-313X.2007.03079.x