TY - JOUR
T1 - Coordinate regulation/localization of the carbohydrate responsive binding protein (ChREBP) by two nuclear export signal sites
T2 - Discovery of a new leucine-rich nuclear export signal site
AU - Fukasawa, Masashi
AU - Ge, Qing
AU - Wynn, R. Max
AU - Ishii, Seiji
AU - Uyeda, Kosaku
N1 - Funding Information:
This work was supported by grants from the Department of Veterans Affairs and by National Institute of Health Grant DK063948. We thank Dr. Yuh M. Chook (Univ. Texas Southwestern Med. Cent., Dallas, TX) for the hCRM1 DNA and her valuable discussion.
PY - 2010/1/8
Y1 - 2010/1/8
N2 - Carbohydrate response element binding protein (ChREBP) is responsible for conversion of dietary carbohydrate to storage fat in liver by coordinating expression of the enzymes that channel glycolytic pyruvate into lipogenesis. The activation of ChREBP in response to high glucose is nuclear localization and transcription, and the inactivation of ChREBP under low glucose involves export from the nucleus to the cytosol. Here we report a new nuclear export signal site ("NES1") of ChREBP. Together these signals provide ChREBP with two NES sequences, both the previously reported NES2 and now the new NES1 coordinate to interact together with CRM1 (exportin) for nuclear export of the carbohydrate response element binding protein.
AB - Carbohydrate response element binding protein (ChREBP) is responsible for conversion of dietary carbohydrate to storage fat in liver by coordinating expression of the enzymes that channel glycolytic pyruvate into lipogenesis. The activation of ChREBP in response to high glucose is nuclear localization and transcription, and the inactivation of ChREBP under low glucose involves export from the nucleus to the cytosol. Here we report a new nuclear export signal site ("NES1") of ChREBP. Together these signals provide ChREBP with two NES sequences, both the previously reported NES2 and now the new NES1 coordinate to interact together with CRM1 (exportin) for nuclear export of the carbohydrate response element binding protein.
KW - Carbohydrate responsive element binding protein
KW - Glucose signals and 14-3-3
KW - Lipogenesis
KW - Nuclear import/export signal sites
KW - Transcription factor
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U2 - 10.1016/j.bbrc.2009.11.115
DO - 10.1016/j.bbrc.2009.11.115
M3 - Article
C2 - 20025850
AN - SCOPUS:73449089383
SN - 0006-291X
VL - 391
SP - 1166
EP - 1169
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -