Coordination of pancreatic HCO3 - secretion by protein-protein interaction between membrane transporters

Min Goo Lee, Wooin Ahn, Jin Ah Lee, Joo Young Kim, Joo Young Choi, Orson W. Moe, Sharon L. Milgram, Shmuel Muallem, Kyung Hwan Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Increasing evidence suggests that protein-protein interaction is essential in many biological processes including epithelial transport. In this report, we discuss the significance of protein interactions to HCO3 - secretion in pancreatic duct cells. In pancreatic ducts HCO3 - secretion is mediated by cystic fibrosis transmembrane conductance regulator (CFTR) activated luminal Cl-/HCO3 -exchange activity and HCO3 - absorption is achieved by Na+-dependent mechanisms including Na+/H+ exchanger 3 (NHE3). We found biochemical and functional association between CFTR and NHE3. In addition, protein binding through PDZ modules is needed for this regulatory interaction. CFTR affected NHE3 activities in two ways. Acutely, CFTR augmented the cAMP-dependent inhibition of NHE3. In a chronic mechanism, CFTR increases the luminal expression of Na+/H+ exchange in pancreatic duct cells. These findings reveal that protein complexes in the plasma membrane of pancreatic duct cells are highly organized for efficient HCO3 - secretion.

Original languageEnglish (US)
Pages (from-to)203-206
Number of pages4
JournalJournal of the Pancreas
Volume2
Issue number4
StatePublished - Jul 2001

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Cystic Fibrosis Transmembrane Conductance Regulator
Membrane Transport Proteins
Sodium-Hydrogen Antiporter
Pancreatic Ducts
Proteins
Biological Phenomena
Protein Binding
Cell Membrane

Keywords

  • Bicarbonates
  • Cystic fibrosis transmembrane conductance regulator
  • Pancreas
  • Protein binding
  • Sodium-hydrogen antiporter

ASJC Scopus subject areas

  • Endocrinology
  • Gastroenterology

Cite this

Lee, M. G., Ahn, W., Lee, J. A., Kim, J. Y., Choi, J. Y., Moe, O. W., ... Kim, K. H. (2001). Coordination of pancreatic HCO3 - secretion by protein-protein interaction between membrane transporters. Journal of the Pancreas, 2(4), 203-206.

Coordination of pancreatic HCO3 - secretion by protein-protein interaction between membrane transporters. / Lee, Min Goo; Ahn, Wooin; Lee, Jin Ah; Kim, Joo Young; Choi, Joo Young; Moe, Orson W.; Milgram, Sharon L.; Muallem, Shmuel; Kim, Kyung Hwan.

In: Journal of the Pancreas, Vol. 2, No. 4, 07.2001, p. 203-206.

Research output: Contribution to journalArticle

Lee, MG, Ahn, W, Lee, JA, Kim, JY, Choi, JY, Moe, OW, Milgram, SL, Muallem, S & Kim, KH 2001, 'Coordination of pancreatic HCO3 - secretion by protein-protein interaction between membrane transporters', Journal of the Pancreas, vol. 2, no. 4, pp. 203-206.
Lee, Min Goo ; Ahn, Wooin ; Lee, Jin Ah ; Kim, Joo Young ; Choi, Joo Young ; Moe, Orson W. ; Milgram, Sharon L. ; Muallem, Shmuel ; Kim, Kyung Hwan. / Coordination of pancreatic HCO3 - secretion by protein-protein interaction between membrane transporters. In: Journal of the Pancreas. 2001 ; Vol. 2, No. 4. pp. 203-206.
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AB - Increasing evidence suggests that protein-protein interaction is essential in many biological processes including epithelial transport. In this report, we discuss the significance of protein interactions to HCO3 - secretion in pancreatic duct cells. In pancreatic ducts HCO3 - secretion is mediated by cystic fibrosis transmembrane conductance regulator (CFTR) activated luminal Cl-/HCO3 -exchange activity and HCO3 - absorption is achieved by Na+-dependent mechanisms including Na+/H+ exchanger 3 (NHE3). We found biochemical and functional association between CFTR and NHE3. In addition, protein binding through PDZ modules is needed for this regulatory interaction. CFTR affected NHE3 activities in two ways. Acutely, CFTR augmented the cAMP-dependent inhibition of NHE3. In a chronic mechanism, CFTR increases the luminal expression of Na+/H+ exchange in pancreatic duct cells. These findings reveal that protein complexes in the plasma membrane of pancreatic duct cells are highly organized for efficient HCO3 - secretion.

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