Coordination of pancreatic HCO3- secretion by protein-protein interaction between membrane transporters

Min Goo Lee, Wooin Ahn, Jin Ah Lee, Joo Young Kim, Joo Young Choi, Orson W. Moe, Sharon L. Milgram, Shmuel Muallem, Kyung Hwan Kim

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Increasing evidence suggests that protein-protein interaction is essential in many biological processes including epithelial transport. In this report, we discuss the significance of protein interactions to HCO3- secretion in pancreatic duct cells. In pancreatic ducts HCO3- secretion is mediated by cystic fibrosis transmembrane conductance regulator (CFTR) activated luminal Cl-/HCO3-exchange activity and HCO3- absorption is achieved by Na+-dependent mechanisms including Na+/H+ exchanger 3 (NHE3). We found biochemical and functional association between CFTR and NHE3. In addition, protein binding through PDZ modules is needed for this regulatory interaction. CFTR affected NHE3 activities in two ways. Acutely, CFTR augmented the cAMP-dependent inhibition of NHE3. In a chronic mechanism, CFTR increases the luminal expression of Na+/H+ exchange in pancreatic duct cells. These findings reveal that protein complexes in the plasma membrane of pancreatic duct cells are highly organized for efficient HCO3- secretion.

Original languageEnglish (US)
Pages (from-to)203-206
Number of pages4
JournalJournal of the Pancreas
Volume2
Issue number4
StatePublished - Jul 1 2001

Keywords

  • Bicarbonates
  • Cystic fibrosis transmembrane conductance regulator
  • Pancreas
  • Protein binding
  • Sodium-hydrogen antiporter

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

Fingerprint Dive into the research topics of 'Coordination of pancreatic HCO<sub>3</sub><sup>-</sup> secretion by protein-protein interaction between membrane transporters'. Together they form a unique fingerprint.

  • Cite this

    Lee, M. G., Ahn, W., Lee, J. A., Kim, J. Y., Choi, J. Y., Moe, O. W., Milgram, S. L., Muallem, S., & Kim, K. H. (2001). Coordination of pancreatic HCO3- secretion by protein-protein interaction between membrane transporters. Journal of the Pancreas, 2(4), 203-206.