Corneal opacity in lumican-null mice: Defects in collagen fibril structure and packing in the posterior stroma

S. Chakravarti, W. M. Petroll, J. R. Hassell, J. V. Jester, J. H. Lass, J. Paul, D. E. Birk

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Abstract

Purpose. Gene targeted lumican-null mutants (lum(tm1sc)/lum(tm1sc) have cloudy corneas with abnormally thick collagen fibrils. The purpose of the present study was to analyze the loss of transparency quantitatively and to define the associated corneal collagen fibril and stromal defects. Methods. Backscattering of light, a function of corneal haze and opacification, was determined regionally using in vivo confocal microscopy in lumican-deficient and wild-type control mice. Fibril organization and structure were analyzed using transmission electron microscopy. Biochemical approaches were used to quantify glycosaminoglycan contents. Lumican distribution in the cornea was elucidated immunohistochemically. Results. Compared with control stromas, lumican-deficient stromas displayed a threefold increase in backscattered light with maximal increase confined to the posterior stroma. Confocal microscopy through-focusing (CMTF) measurement profiles also indicated a 40% reduction in stromal thickness in the lumican-null mice. Transmission electron microscopy indicated significant collagen fibril abnormalities in the posterior stroma, with the anterior stroma remaining relatively unremarkable. The lumican-deficient posterior stroma displayed a pronounced increase in fibril diameter, large fibril aggregates, altered fibril packing, and poor lamellar organization. Immunostaining of wild-type corneas demonstrated high concentrations of lumican in the posterior stroma. Biochemical assessment of keratan sulfate (KS) content of whole eyes revealed a 25% reduction in KS content in the lumican-deficient mice. Conclusions. The structural defects and maximum backscattering of light clearly localized to the posterior stroma of lumican-deficient mice. In normal mice, an enrichment of lumican was observed in the posterior stroma compared with that in the anterior stroma. Taken together, these observations indicate a key role for lumican in the posterior stroma in maintaining normal fibril architecture, most likely by regulating fibril assembly and maintaining optimal KS content required for transparency.

Original languageEnglish (US)
Pages (from-to)3365-3373
Number of pages9
JournalInvestigative Ophthalmology and Visual Science
Volume41
Issue number11
StatePublished - 2000

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Corneal Opacity
Collagen
Keratan Sulfate
Cornea
Transmission Electron Microscopy
Light
Confocal Microscopy
Fibril-Associated Collagens
Lumican
Glycosaminoglycans

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Chakravarti, S., Petroll, W. M., Hassell, J. R., Jester, J. V., Lass, J. H., Paul, J., & Birk, D. E. (2000). Corneal opacity in lumican-null mice: Defects in collagen fibril structure and packing in the posterior stroma. Investigative Ophthalmology and Visual Science, 41(11), 3365-3373.

Corneal opacity in lumican-null mice : Defects in collagen fibril structure and packing in the posterior stroma. / Chakravarti, S.; Petroll, W. M.; Hassell, J. R.; Jester, J. V.; Lass, J. H.; Paul, J.; Birk, D. E.

In: Investigative Ophthalmology and Visual Science, Vol. 41, No. 11, 2000, p. 3365-3373.

Research output: Contribution to journalArticle

Chakravarti, S, Petroll, WM, Hassell, JR, Jester, JV, Lass, JH, Paul, J & Birk, DE 2000, 'Corneal opacity in lumican-null mice: Defects in collagen fibril structure and packing in the posterior stroma', Investigative Ophthalmology and Visual Science, vol. 41, no. 11, pp. 3365-3373.
Chakravarti, S. ; Petroll, W. M. ; Hassell, J. R. ; Jester, J. V. ; Lass, J. H. ; Paul, J. ; Birk, D. E. / Corneal opacity in lumican-null mice : Defects in collagen fibril structure and packing in the posterior stroma. In: Investigative Ophthalmology and Visual Science. 2000 ; Vol. 41, No. 11. pp. 3365-3373.
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abstract = "Purpose. Gene targeted lumican-null mutants (lum(tm1sc)/lum(tm1sc) have cloudy corneas with abnormally thick collagen fibrils. The purpose of the present study was to analyze the loss of transparency quantitatively and to define the associated corneal collagen fibril and stromal defects. Methods. Backscattering of light, a function of corneal haze and opacification, was determined regionally using in vivo confocal microscopy in lumican-deficient and wild-type control mice. Fibril organization and structure were analyzed using transmission electron microscopy. Biochemical approaches were used to quantify glycosaminoglycan contents. Lumican distribution in the cornea was elucidated immunohistochemically. Results. Compared with control stromas, lumican-deficient stromas displayed a threefold increase in backscattered light with maximal increase confined to the posterior stroma. Confocal microscopy through-focusing (CMTF) measurement profiles also indicated a 40{\%} reduction in stromal thickness in the lumican-null mice. Transmission electron microscopy indicated significant collagen fibril abnormalities in the posterior stroma, with the anterior stroma remaining relatively unremarkable. The lumican-deficient posterior stroma displayed a pronounced increase in fibril diameter, large fibril aggregates, altered fibril packing, and poor lamellar organization. Immunostaining of wild-type corneas demonstrated high concentrations of lumican in the posterior stroma. Biochemical assessment of keratan sulfate (KS) content of whole eyes revealed a 25{\%} reduction in KS content in the lumican-deficient mice. Conclusions. The structural defects and maximum backscattering of light clearly localized to the posterior stroma of lumican-deficient mice. In normal mice, an enrichment of lumican was observed in the posterior stroma compared with that in the anterior stroma. Taken together, these observations indicate a key role for lumican in the posterior stroma in maintaining normal fibril architecture, most likely by regulating fibril assembly and maintaining optimal KS content required for transparency.",
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T1 - Corneal opacity in lumican-null mice

T2 - Defects in collagen fibril structure and packing in the posterior stroma

AU - Chakravarti, S.

AU - Petroll, W. M.

AU - Hassell, J. R.

AU - Jester, J. V.

AU - Lass, J. H.

AU - Paul, J.

AU - Birk, D. E.

PY - 2000

Y1 - 2000

N2 - Purpose. Gene targeted lumican-null mutants (lum(tm1sc)/lum(tm1sc) have cloudy corneas with abnormally thick collagen fibrils. The purpose of the present study was to analyze the loss of transparency quantitatively and to define the associated corneal collagen fibril and stromal defects. Methods. Backscattering of light, a function of corneal haze and opacification, was determined regionally using in vivo confocal microscopy in lumican-deficient and wild-type control mice. Fibril organization and structure were analyzed using transmission electron microscopy. Biochemical approaches were used to quantify glycosaminoglycan contents. Lumican distribution in the cornea was elucidated immunohistochemically. Results. Compared with control stromas, lumican-deficient stromas displayed a threefold increase in backscattered light with maximal increase confined to the posterior stroma. Confocal microscopy through-focusing (CMTF) measurement profiles also indicated a 40% reduction in stromal thickness in the lumican-null mice. Transmission electron microscopy indicated significant collagen fibril abnormalities in the posterior stroma, with the anterior stroma remaining relatively unremarkable. The lumican-deficient posterior stroma displayed a pronounced increase in fibril diameter, large fibril aggregates, altered fibril packing, and poor lamellar organization. Immunostaining of wild-type corneas demonstrated high concentrations of lumican in the posterior stroma. Biochemical assessment of keratan sulfate (KS) content of whole eyes revealed a 25% reduction in KS content in the lumican-deficient mice. Conclusions. The structural defects and maximum backscattering of light clearly localized to the posterior stroma of lumican-deficient mice. In normal mice, an enrichment of lumican was observed in the posterior stroma compared with that in the anterior stroma. Taken together, these observations indicate a key role for lumican in the posterior stroma in maintaining normal fibril architecture, most likely by regulating fibril assembly and maintaining optimal KS content required for transparency.

AB - Purpose. Gene targeted lumican-null mutants (lum(tm1sc)/lum(tm1sc) have cloudy corneas with abnormally thick collagen fibrils. The purpose of the present study was to analyze the loss of transparency quantitatively and to define the associated corneal collagen fibril and stromal defects. Methods. Backscattering of light, a function of corneal haze and opacification, was determined regionally using in vivo confocal microscopy in lumican-deficient and wild-type control mice. Fibril organization and structure were analyzed using transmission electron microscopy. Biochemical approaches were used to quantify glycosaminoglycan contents. Lumican distribution in the cornea was elucidated immunohistochemically. Results. Compared with control stromas, lumican-deficient stromas displayed a threefold increase in backscattered light with maximal increase confined to the posterior stroma. Confocal microscopy through-focusing (CMTF) measurement profiles also indicated a 40% reduction in stromal thickness in the lumican-null mice. Transmission electron microscopy indicated significant collagen fibril abnormalities in the posterior stroma, with the anterior stroma remaining relatively unremarkable. The lumican-deficient posterior stroma displayed a pronounced increase in fibril diameter, large fibril aggregates, altered fibril packing, and poor lamellar organization. Immunostaining of wild-type corneas demonstrated high concentrations of lumican in the posterior stroma. Biochemical assessment of keratan sulfate (KS) content of whole eyes revealed a 25% reduction in KS content in the lumican-deficient mice. Conclusions. The structural defects and maximum backscattering of light clearly localized to the posterior stroma of lumican-deficient mice. In normal mice, an enrichment of lumican was observed in the posterior stroma compared with that in the anterior stroma. Taken together, these observations indicate a key role for lumican in the posterior stroma in maintaining normal fibril architecture, most likely by regulating fibril assembly and maintaining optimal KS content required for transparency.

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