Cortisol Modification of HeLa 65 Alkaline Phosphatase: Decreased Phosphate Content of the Induced Enzyme

Kirstan L. BAZZELL, Gary PRICE, Sylvia TU, Martin GRIFFIN, Rody COX, Nimai GHOSH

Research output: Contribution to journalArticle

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Abstract

Alkaline phosphatase activity of HeLa cells is increased 5–20‐fold during growth in medium with cortisol. The increase in enzyme activity is due to an enhanced catalytic efficiency rather than an increase in alkaline phosphatase protein in induced cells. In the present study the chemical composition of control and induced forms of alkaline phosphatase were investigated to determine the enzyme modification that may be responsible for the increased catalytic activity. HeLa alkaline phosphatase is a phosphoprotein and the induced form of the enzyme has approximately one‐half of the phosphate residues associated with control enzyme. The decrease in phosphate residues of the enzyme apparently alters its catalytic activity. Other chemical components of purified alkaline phosphatase from control and induced cells are similar; these include sialic acid, hexosamine and sulfhydryl residues.

Original languageEnglish (US)
Pages (from-to)493-499
Number of pages7
JournalEuropean Journal of Biochemistry
Volume61
Issue number2
DOIs
StatePublished - 1976

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Alkaline Phosphatase
Hydrocortisone
Phosphates
Enzymes
Catalyst activity
Hexosamines
Phosphoproteins
Enzyme activity
N-Acetylneuraminic Acid
HeLa Cells
Growth
Chemical analysis
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cortisol Modification of HeLa 65 Alkaline Phosphatase : Decreased Phosphate Content of the Induced Enzyme. / BAZZELL, Kirstan L.; PRICE, Gary; TU, Sylvia; GRIFFIN, Martin; COX, Rody; GHOSH, Nimai.

In: European Journal of Biochemistry, Vol. 61, No. 2, 1976, p. 493-499.

Research output: Contribution to journalArticle

BAZZELL, Kirstan L. ; PRICE, Gary ; TU, Sylvia ; GRIFFIN, Martin ; COX, Rody ; GHOSH, Nimai. / Cortisol Modification of HeLa 65 Alkaline Phosphatase : Decreased Phosphate Content of the Induced Enzyme. In: European Journal of Biochemistry. 1976 ; Vol. 61, No. 2. pp. 493-499.
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