Counting and sizing of epidermal cells in normal human skin

P. R. Bergstresser, R. J. Pariser, J. R. Taylor

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

During keratinocyte maturation, individual cells undergo an orderly succession of biochemical and structural changes. In certain skin disorders alterations in keratinocyte numbers, volumes, and epidermal skin thickness occur. To assess such alterations and to provide base line values for normal human epidermis, a computer assisted histologic technique was developed. Skin biopsies were taken from normal skin on the forearm, back and thigh of 6 adult men. Whole specimens of epidermis were separated from the dermis with collagenase, fixed, stained, and mounted for microscopic examination. From the three dimensional coordinates of epidermal nuclei, epidermal cell volumes, surface density of epidermal cells, and epidermal thickness were determined. Measurement of cell volumes in this way compared favorably with electronic cell sizing of disaggregated epidermal cells in matched samples. The mean densities of nucleated cells per 104 μ2 surface area were 452, 483, and 487 for the forearm, back and thigh respectively. This technique will be used to make similar assessments in disorders of abnormal keratinocyte maturation.

Original languageEnglish (US)
Pages (from-to)280-284
Number of pages5
JournalJournal of Investigative Dermatology
Volume70
Issue number5
StatePublished - 1978

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Skin
Keratinocytes
Thigh
Forearm
Epidermis
Cell Count
Cell Nucleus Size
Histological Techniques
Biopsy
Collagenases
Dermis
Cell Size
Microscopic examination
Reference Values

ASJC Scopus subject areas

  • Dermatology

Cite this

Bergstresser, P. R., Pariser, R. J., & Taylor, J. R. (1978). Counting and sizing of epidermal cells in normal human skin. Journal of Investigative Dermatology, 70(5), 280-284.

Counting and sizing of epidermal cells in normal human skin. / Bergstresser, P. R.; Pariser, R. J.; Taylor, J. R.

In: Journal of Investigative Dermatology, Vol. 70, No. 5, 1978, p. 280-284.

Research output: Contribution to journalArticle

Bergstresser, PR, Pariser, RJ & Taylor, JR 1978, 'Counting and sizing of epidermal cells in normal human skin', Journal of Investigative Dermatology, vol. 70, no. 5, pp. 280-284.
Bergstresser, P. R. ; Pariser, R. J. ; Taylor, J. R. / Counting and sizing of epidermal cells in normal human skin. In: Journal of Investigative Dermatology. 1978 ; Vol. 70, No. 5. pp. 280-284.
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