Cryopreserved human fetal pancreas: A source of insulin-producing tissue?

Ingemar Dawidson, Randall Simonsen, Shanti Aggarwal, Laura Coorpender, Kenneth Diller, Ray Rajotte, Philip Raskin, Helen Redman, Julio Rosenstock

Research output: Contribution to journalArticle

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Abstract

Human fetal pancreata (HFP) were obtained from dilatation and extraction aborted fetuses of 11-18 weeks' gestation. The pancreas was excised under sterile conditions and kept in culture medium at 4 °C, prior to stepwise digestion into 50- to 150-μm fragments. The fragmented pieces were allowed to sediment by gravity, then transferred to tissue culture for 24-48 h, and cryopreserved. The freeze-thaw protocol used stepwise equilibration with dimethyl sulfoxide, nucleation of the sample at -10 °C, and a slow cooling rate of 0.25 °C/min to -40 °C, followed by submersion in liquid nitrogen (-196 °C). Rapid thawing at 300 °C/min from -196 °C was employed. Both fresh and frozen-thawed HFP fragments appeared viable as judged by light and electron microscopy, and secreted insulin in a perifusion system upon stimulation with glucose (28 mM) and theophylline (10 mM) or glucose (2.8 mM) and theophylline (10 mM). Six patients with Type I insulin-dependent diabetes mellitus, already requiring immunosuppression for a kidney transplant, had intraportal injection of 20 cryopreserved-thawed and pooled HFP fragments. Up to the 1-year post-transplant follow-up, there has been no evidence of in vivo insulin or C-peptide production. The usefulness of cryopreserved human fetal pancreata as a source of insulin-producing tissue for diabetic patients, therefore, remains to be demonstrated.

Original languageEnglish (US)
Pages (from-to)83-93
Number of pages11
JournalCryobiology
Volume25
Issue number2
DOIs
StatePublished - 1988

Fingerprint

pancreas
Pancreas
insulin
Insulin
Tissue
Transplants
Theophylline
theophylline
insulin-dependent diabetes mellitus
Type 1 Diabetes Mellitus
Glucose
Tissue culture
Thawing
C-Peptide
Aborted Fetus
Liquid nitrogen
Medical problems
Dimethyl Sulfoxide
kidney transplant
glucose

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Dawidson, I., Simonsen, R., Aggarwal, S., Coorpender, L., Diller, K., Rajotte, R., ... Rosenstock, J. (1988). Cryopreserved human fetal pancreas: A source of insulin-producing tissue? Cryobiology, 25(2), 83-93. https://doi.org/10.1016/0011-2240(88)90001-6

Cryopreserved human fetal pancreas : A source of insulin-producing tissue? / Dawidson, Ingemar; Simonsen, Randall; Aggarwal, Shanti; Coorpender, Laura; Diller, Kenneth; Rajotte, Ray; Raskin, Philip; Redman, Helen; Rosenstock, Julio.

In: Cryobiology, Vol. 25, No. 2, 1988, p. 83-93.

Research output: Contribution to journalArticle

Dawidson, I, Simonsen, R, Aggarwal, S, Coorpender, L, Diller, K, Rajotte, R, Raskin, P, Redman, H & Rosenstock, J 1988, 'Cryopreserved human fetal pancreas: A source of insulin-producing tissue?', Cryobiology, vol. 25, no. 2, pp. 83-93. https://doi.org/10.1016/0011-2240(88)90001-6
Dawidson I, Simonsen R, Aggarwal S, Coorpender L, Diller K, Rajotte R et al. Cryopreserved human fetal pancreas: A source of insulin-producing tissue? Cryobiology. 1988;25(2):83-93. https://doi.org/10.1016/0011-2240(88)90001-6
Dawidson, Ingemar ; Simonsen, Randall ; Aggarwal, Shanti ; Coorpender, Laura ; Diller, Kenneth ; Rajotte, Ray ; Raskin, Philip ; Redman, Helen ; Rosenstock, Julio. / Cryopreserved human fetal pancreas : A source of insulin-producing tissue?. In: Cryobiology. 1988 ; Vol. 25, No. 2. pp. 83-93.
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