Crystal structure of a type III pantothenate kinase: Insight into the mechanism of an essential coenzyme A biosynthetic enzyme universally distributed in bacteria

Kun Yang, Yvonne Eyobo, Leisl A. Brand, Dariusz Martynowski, Diana Tomchick, Erick Strauss, Hong Zhang

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Pantothenate kinase (PanK) catalyzes the first step in the five-step universal pathway of coenzyme A (CoA) biosynthesis, a key transformation that generally also regulates the intracellular concentration of CoA through feedback inhibition. A novel PanK protein encoded by the gene coaX was recently identified that is distinct from the previously characterized type I PanK (exemplified by the Escherichia coli coaA-encoded PanK protein) and type II eukaryotic PanKs and is not inhibited by CoA or its thioesters. This type III PanK, or PanK-III, is widely distributed in the bacterial kingdom and accounts for the only known PanK in many pathogenic species, such as Helicobacter pylori, Bordetella pertussis, and Pseudomonas aeruginosa. Here we report the first crystal structure of a type III PanK, the enzyme from Thermotoga maritima (PanKTm), solved at 2.0-Å resolution. The structure of PanKTm reveals that type III PanKs belong to the acetate and sugar kinase/heat shock protein 70/actin (ASKHA) protein superfamily and that they retain the highly conserved active site motifs common to all members of this superfamily. Comparative structural analysis of the PanKTm active site configuration and mutagenesis of three highly conserved active site aspartates identify these residues as critical for PanK-III catalysis. Furthermore, the analysis also provides an explanation for the lack of CoA feedback inhibition by the enzyme. Since PanK-III adopts a different structural fold from that of the E. coli PanK-which is a member of the "P-loop kinase" superfamily-this finding represents yet another example of convergent evolution of the same biological function from a different protein ancestor.

Original languageEnglish (US)
Pages (from-to)5532-5540
Number of pages9
JournalJournal of Bacteriology
Volume188
Issue number15
DOIs
StatePublished - Aug 2006

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Coenzyme A
Bacteria
Enzymes
Thermotoga maritima
Catalytic Domain
Proteins
pantothenate kinase
Acetate Kinase
Biological Evolution
Escherichia coli
Bordetella pertussis
HSP70 Heat-Shock Proteins
Catalysis
Aspartic Acid
Helicobacter pylori
Mutagenesis
Pseudomonas aeruginosa
Actins
Phosphotransferases

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Crystal structure of a type III pantothenate kinase : Insight into the mechanism of an essential coenzyme A biosynthetic enzyme universally distributed in bacteria. / Yang, Kun; Eyobo, Yvonne; Brand, Leisl A.; Martynowski, Dariusz; Tomchick, Diana; Strauss, Erick; Zhang, Hong.

In: Journal of Bacteriology, Vol. 188, No. 15, 08.2006, p. 5532-5540.

Research output: Contribution to journalArticle

Yang, Kun ; Eyobo, Yvonne ; Brand, Leisl A. ; Martynowski, Dariusz ; Tomchick, Diana ; Strauss, Erick ; Zhang, Hong. / Crystal structure of a type III pantothenate kinase : Insight into the mechanism of an essential coenzyme A biosynthetic enzyme universally distributed in bacteria. In: Journal of Bacteriology. 2006 ; Vol. 188, No. 15. pp. 5532-5540.
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