Crystallization and preliminary X-ray studies of ornithine decarboxylase from Trypanosoma brucei

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14 Citations (Scopus)

Abstract

Trypanosoma brucei ornithine decarboxylase, expressed and purified from E. coli, has been crystallized by the vapor diffusion method using PEG 3350 as a precipitant. The crystals belong to the monoclinic space group P21 and have cell constants of a = 66.3 Å, b = 151.8 Å, c = 83.7 Å, and β = 101.2°. While larger crystals are twinned, smaller crystals (0.4 x 0.3 x 0.05 mm3) are single.

Original languageEnglish (US)
Pages (from-to)272-273
Number of pages2
JournalProteins: Structure, Function and Genetics
Volume24
Issue number2
DOIs
StatePublished - 1996

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Trypanosoma brucei brucei
Ornithine Decarboxylase
Crystallization
X-Rays
Escherichia coli
X rays
Crystals
Vapors
polyethylene glycol 3350

Keywords

  • drug design
  • polyamines
  • pyridoxal phosphate

ASJC Scopus subject areas

  • Genetics
  • Structural Biology
  • Biochemistry

Cite this

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title = "Crystallization and preliminary X-ray studies of ornithine decarboxylase from Trypanosoma brucei",
abstract = "Trypanosoma brucei ornithine decarboxylase, expressed and purified from E. coli, has been crystallized by the vapor diffusion method using PEG 3350 as a precipitant. The crystals belong to the monoclinic space group P21 and have cell constants of a = 66.3 {\AA}, b = 151.8 {\AA}, c = 83.7 {\AA}, and β = 101.2°. While larger crystals are twinned, smaller crystals (0.4 x 0.3 x 0.05 mm3) are single.",
keywords = "drug design, polyamines, pyridoxal phosphate",
author = "Grishin, {Nick V.} and Osterman, {Andrei L.} and Goldsmith, {Elizabeth J.} and Phillips, {Margaret A.}",
year = "1996",
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T1 - Crystallization and preliminary X-ray studies of ornithine decarboxylase from Trypanosoma brucei

AU - Grishin, Nick V.

AU - Osterman, Andrei L.

AU - Goldsmith, Elizabeth J.

AU - Phillips, Margaret A.

PY - 1996

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AB - Trypanosoma brucei ornithine decarboxylase, expressed and purified from E. coli, has been crystallized by the vapor diffusion method using PEG 3350 as a precipitant. The crystals belong to the monoclinic space group P21 and have cell constants of a = 66.3 Å, b = 151.8 Å, c = 83.7 Å, and β = 101.2°. While larger crystals are twinned, smaller crystals (0.4 x 0.3 x 0.05 mm3) are single.

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KW - pyridoxal phosphate

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