Crystallization of a complex between a novel C-terminal transmitter, HPt domain, of the anaerobic sensor kinase ArcB and the chemotaxis response regulator CheY

Masato Kato, Takeshi Mizuno, Toshio Hakoshima

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The histidine-containing phosphotransfer (HPt) domain at the C-terminus of the anaerobic sensor kinase ArcB has been cocrystallized with the chemotaxis response regulator CheY by a hanging-drop vapor-diffusion method. Crystals belong to space group P212121 with unit-cell dimensions a = 55.32, b = 76.29 and c = 83.89 Å, with one molecule in the crystallographic asymmetric unit. The crystals diffract to 2.7 Å resolution. This is the first crystallization of a protein-protein complex formed by a transmitter domain of sensor kinase and a receiver domain of response regulator in the two-component signal-transduction system.

Original languageEnglish (US)
Pages (from-to)140-142
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume54
Issue number1
DOIs
StatePublished - Jan 1 1998

Fingerprint

histidine
regulators
Chemotaxis
Crystallization
Histidine
transmitters
Transmitters
Phosphotransferases
crystallization
Signal transduction
Crystals
sensors
Sensors
proteins
Signal Transduction
Proteins
Vapors
Molecules
crystals
receivers

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

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abstract = "The histidine-containing phosphotransfer (HPt) domain at the C-terminus of the anaerobic sensor kinase ArcB has been cocrystallized with the chemotaxis response regulator CheY by a hanging-drop vapor-diffusion method. Crystals belong to space group P212121 with unit-cell dimensions a = 55.32, b = 76.29 and c = 83.89 {\AA}, with one molecule in the crystallographic asymmetric unit. The crystals diffract to 2.7 {\AA} resolution. This is the first crystallization of a protein-protein complex formed by a transmitter domain of sensor kinase and a receiver domain of response regulator in the two-component signal-transduction system.",
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