Abstract

Plasmacytoid dendritic cells (pDCs) are vital to antiviral defense, directing immune responses via secretion of huge concentrations of IFN-α. These cells are critical in protecting the lung against clinically relevant respiratory viruses, particularly influenza (Flu), a virus responsible for substantial worldwide morbidity and mortality. How pDC responses to such viral pathogens are regulated, however, is poorly understood in humans. Using an unbiased approach of gene chip analysis, we discovered that Flu significantly affects metabolism in primary human pDCs. We demonstrate that Flu and RV, another common respiratory virus, induce glycolysis in pDCs and that this metabolic pathway regulates pDC antiviral functions, including IFN-α production and phenotypic maturation. Intranasal vaccination of human volunteers with live influenza virus also increases glycolysis in circulating pDCs, highlighting a previously unrecognized potential role for metabolism in regulating pDC immune responses to viral infections in humans.

Original languageEnglish (US)
Pages (from-to)2004-2009
Number of pages6
JournalJournal of Immunology
Volume196
Issue number5
DOIs
StatePublished - Mar 1 2016

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Glycolysis
Dendritic Cells
Antiviral Agents
Orthomyxoviridae
Viruses
Virus Diseases
Metabolic Networks and Pathways
Oligonucleotide Array Sequence Analysis
Volunteers
Vaccination
Morbidity
Lung
Mortality

ASJC Scopus subject areas

  • Immunology

Cite this

Cutting Edge : Critical role of glycolysis in human plasmacytoid dendritic cell antiviral responses. / Bajwa, Gagan; DeBerardinis, Ralph J.; Shao, Baomei; Hall, Brian; Farrar, J. David; Gill, Michelle A.

In: Journal of Immunology, Vol. 196, No. 5, 01.03.2016, p. 2004-2009.

Research output: Contribution to journalArticle

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