Cyclic di-GMP-mediated repression of swarming motility by Pseudomonas aeruginosa PA14 Requires the MotAB stator

S. L. Kuchma, N. J. Delalez, L. M. Filkins, E. A. Snavely, J. P. Armitage, G. A. O'Toole

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The second messenger cyclic diguanylate (c-di-GMP) plays a critical role in the regulation of motility. In Pseudomonas aeruginosa PA14, c-di-GMP inversely controls biofilm formation and surface swarming motility, with high levels of this dinucleotide signal stimulating biofilm formation and repressing swarming. P. aeruginosa encodes two stator complexes, MotAB and MotCD, that participate in the function of its single polar flagellum. Here we show that the repression of swarming motility requires a functional MotAB stator complex. Mutating the motAB genes restores swarming motility to a strain with artificially elevated levels of c-di-GMP as well as stimulates swarming in the wild-type strain, while overexpression of MotA from a plasmid represses swarming motility. Using point mutations in MotA and the FliG rotor protein of the motor supports the conclusion that MotA-FliG interactions are critical for c-di-GMP-mediated swarming inhibition. Finally, we show that high c-di-GMP levels affect the localization of a green fluorescent protein (GFP)-MotD fusion, indicating a mechanism whereby this second messenger has an impact on MotCD function. We propose that when c-di-GMP level is high, the MotAB stator can displace MotCD from the motor, thereby affecting motor function. Our data suggest a newly identified means of c-di-GMP-mediated control of surface motility, perhaps conserved among Pseudomonas, Xanthomonas, and other organisms that encode two stator systems.

Original languageEnglish (US)
Pages (from-to)420-430
Number of pages11
JournalJournal of Bacteriology
Volume197
Issue number3
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

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Pseudomonas aeruginosa
Second Messenger Systems
Biofilms
Xanthomonas
Flagella
Pseudomonas
Green Fluorescent Proteins
bis(3',5')-cyclic diguanylic acid
Point Mutation
Plasmids
Genes
Proteins

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Cyclic di-GMP-mediated repression of swarming motility by Pseudomonas aeruginosa PA14 Requires the MotAB stator. / Kuchma, S. L.; Delalez, N. J.; Filkins, L. M.; Snavely, E. A.; Armitage, J. P.; O'Toole, G. A.

In: Journal of Bacteriology, Vol. 197, No. 3, 01.01.2015, p. 420-430.

Research output: Contribution to journalArticle

Kuchma, S. L. ; Delalez, N. J. ; Filkins, L. M. ; Snavely, E. A. ; Armitage, J. P. ; O'Toole, G. A. / Cyclic di-GMP-mediated repression of swarming motility by Pseudomonas aeruginosa PA14 Requires the MotAB stator. In: Journal of Bacteriology. 2015 ; Vol. 197, No. 3. pp. 420-430.
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