D2d, a D-End class I gene: Tissue expression and alternative processing of the pre-mRNA

Marylynne Hedley, James Forman, Philip W. Tucker

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The H-2D region in the major histocompatibility complex (MHC) of the BALB/c mouse includes at least five class I genes: Dd, D2d, D3d, D4d, and Ld. The pattern of expression and functions of the D2d, D3d, and D4d genes are not known. To examine the expression of D2d we obtained mouse L cells transfected with a wildtype D2d gene or an exon shufled D2d/D2d/Dd gene that contained exons encoding the α1 and α2 domains of D2d and the α3 domain of H-2Dd. Analysis of the mRNA in transfected cells suggested that two forms of the message were generated at approximately equal abundance by alternative splicing. Several tissues were analyzed and shown to express both forms of the D2d mRNA although the highest levels were found in lymphoid organs. Tumor lines also expressed D2d mRNA but exhibited differing ratios of alternative versus normally spliced message. This ratio was also affected by treatment of cells with Con A supernatants that contain interferon or infection with vesicular stomatitis virus (VSV).

Original languageEnglish (US)
Pages (from-to)25-32
Number of pages8
JournalInternational Immunology
Volume2
Issue number1
DOIs
StatePublished - Jan 1 1990

Keywords

  • Alternative processing
  • D2d
  • Pre-mRNA
  • Tissue expression

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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