Deep reflection-mode photoacoustic imaging of biological tissue

Kwang Hyun Song, Lihong V. Wang

Research output: Contribution to journalArticlepeer-review

172 Scopus citations


A reflection-mode photoacoustic (PA) imaging system was designed and built to image deep structures in biological tissues. We chose near-infrared laser pulses of 804-nm wavelength for PA excitation to achieve deep penetration. To minimize unwanted surface signals, we adopted dark-field ring-shaped illumination. This imaging system employing a 5-MHz spherically focused ultrasonic transducer provides penetration up to 38 mm in chicken breast tissue. At the 19-mm depth, the axial resolution is 144 μm and the transverse resolution is 560 μm. Internal organs of small animals were imaged clearly.

Original languageEnglish (US)
Article number060503
JournalJournal of biomedical optics
Issue number6
StatePublished - 2007


  • Brain cortex
  • Deep imaging
  • Photoacoustic (PA) imaging
  • Reflection-mode imaging
  • Spleen

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering


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