Demonstration of membrane-bound receptors by means of autoradiography

Vinci Mizuhira, Junko Yokofujita, Hidenori Suzuki, Yoshie Sugiura

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The binding sites of membrane-bound receptors such as insulin, acetyl choline, concanavaline A (Con A) are clearly demonstrated with a radioactive isotope (hot RI) incubation after light fixation. This type of autoradiography (ARG) visualizes the correct binding sites with good morphological characteristics. For steroid hormones such as estrogen including vitamine D3, the genomic receptor sites cannot be demonstrated with such samples except the essential specific strong binding affinity in the special granules of eosinophilic leucocytes. We demonstrated the binding sites of 125I-insulin in liver, red blood cells, and heart muscle; of 125I-α-bungarotoxin (bungarotoxin) in rat intercostal muscle motor end-plate, cerebellum, and cerebrum; of 125I- or 3H-Con A receptors on the surface of human red blood cells; and of 3H-estradiol or 3H-moxestrol in the special granules of eosinophilic leucocytes in the rat uterus.

Original languageEnglish (US)
Pages (from-to)687-700
Number of pages14
JournalActa Histochemica et Cytochemica
Volume19
Issue number6
DOIs
StatePublished - 1986

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Physiology
  • Biochemistry
  • Cell Biology

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