Dendritic fibroblasts in three-dimensional collagen matrices

Frederick Grinnell, Chin Han Ho, Elisa Tamariz, David J. Lee, Gabriella Skuta

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

Cell motility determines form and function of multicellular organisms. Most studies on fibroblast motility have been carried out using cells on the surfaces of culture dishes. In situ, however, the environment for fibroblasts is the three-dimensional extracellular matrix. In the current research, we studied the morphology and motility of human fibroblasts embedded in floating collagen matrices at a cell density below that required for global matrix remodeling (i.e., contraction). Under these conditions, cells were observed to project and retract a dendritic network of extensions. These extensions contained microtubule cores with actin concentrated at the tips resembling growth cones. Platelet-derived growth factor promoted formation of the network; lysophosphatidic acid stimulated its retraction in a Rho and Rho kinase-dependent manner. The dendritic network also supported metabolic coupling between cells. We suggest that the dendritic network provides a mechanism by which fibroblasts explore and become interconnected to each other in three-dimensional space.

Original languageEnglish (US)
Pages (from-to)384-395
Number of pages12
JournalMolecular Biology of the Cell
Volume14
Issue number2
DOIs
StatePublished - Feb 1 2003

Fingerprint

Collagen
Fibroblasts
rho-Associated Kinases
Growth Cones
Platelet-Derived Growth Factor
Microtubules
Cell Movement
Extracellular Matrix
Actins
Cell Count
Research

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Dendritic fibroblasts in three-dimensional collagen matrices. / Grinnell, Frederick; Ho, Chin Han; Tamariz, Elisa; Lee, David J.; Skuta, Gabriella.

In: Molecular Biology of the Cell, Vol. 14, No. 2, 01.02.2003, p. 384-395.

Research output: Contribution to journalArticle

Grinnell, Frederick ; Ho, Chin Han ; Tamariz, Elisa ; Lee, David J. ; Skuta, Gabriella. / Dendritic fibroblasts in three-dimensional collagen matrices. In: Molecular Biology of the Cell. 2003 ; Vol. 14, No. 2. pp. 384-395.
@article{0c86400db6e44640b9d5da143ccdd752,
title = "Dendritic fibroblasts in three-dimensional collagen matrices",
abstract = "Cell motility determines form and function of multicellular organisms. Most studies on fibroblast motility have been carried out using cells on the surfaces of culture dishes. In situ, however, the environment for fibroblasts is the three-dimensional extracellular matrix. In the current research, we studied the morphology and motility of human fibroblasts embedded in floating collagen matrices at a cell density below that required for global matrix remodeling (i.e., contraction). Under these conditions, cells were observed to project and retract a dendritic network of extensions. These extensions contained microtubule cores with actin concentrated at the tips resembling growth cones. Platelet-derived growth factor promoted formation of the network; lysophosphatidic acid stimulated its retraction in a Rho and Rho kinase-dependent manner. The dendritic network also supported metabolic coupling between cells. We suggest that the dendritic network provides a mechanism by which fibroblasts explore and become interconnected to each other in three-dimensional space.",
author = "Frederick Grinnell and Ho, {Chin Han} and Elisa Tamariz and Lee, {David J.} and Gabriella Skuta",
year = "2003",
month = "2",
day = "1",
doi = "10.1091/mbc.E02-08-0493",
language = "English (US)",
volume = "14",
pages = "384--395",
journal = "Molecular Biology of the Cell",
issn = "1059-1524",
publisher = "American Society for Cell Biology",
number = "2",

}

TY - JOUR

T1 - Dendritic fibroblasts in three-dimensional collagen matrices

AU - Grinnell, Frederick

AU - Ho, Chin Han

AU - Tamariz, Elisa

AU - Lee, David J.

AU - Skuta, Gabriella

PY - 2003/2/1

Y1 - 2003/2/1

N2 - Cell motility determines form and function of multicellular organisms. Most studies on fibroblast motility have been carried out using cells on the surfaces of culture dishes. In situ, however, the environment for fibroblasts is the three-dimensional extracellular matrix. In the current research, we studied the morphology and motility of human fibroblasts embedded in floating collagen matrices at a cell density below that required for global matrix remodeling (i.e., contraction). Under these conditions, cells were observed to project and retract a dendritic network of extensions. These extensions contained microtubule cores with actin concentrated at the tips resembling growth cones. Platelet-derived growth factor promoted formation of the network; lysophosphatidic acid stimulated its retraction in a Rho and Rho kinase-dependent manner. The dendritic network also supported metabolic coupling between cells. We suggest that the dendritic network provides a mechanism by which fibroblasts explore and become interconnected to each other in three-dimensional space.

AB - Cell motility determines form and function of multicellular organisms. Most studies on fibroblast motility have been carried out using cells on the surfaces of culture dishes. In situ, however, the environment for fibroblasts is the three-dimensional extracellular matrix. In the current research, we studied the morphology and motility of human fibroblasts embedded in floating collagen matrices at a cell density below that required for global matrix remodeling (i.e., contraction). Under these conditions, cells were observed to project and retract a dendritic network of extensions. These extensions contained microtubule cores with actin concentrated at the tips resembling growth cones. Platelet-derived growth factor promoted formation of the network; lysophosphatidic acid stimulated its retraction in a Rho and Rho kinase-dependent manner. The dendritic network also supported metabolic coupling between cells. We suggest that the dendritic network provides a mechanism by which fibroblasts explore and become interconnected to each other in three-dimensional space.

UR - http://www.scopus.com/inward/record.url?scp=0037328762&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037328762&partnerID=8YFLogxK

U2 - 10.1091/mbc.E02-08-0493

DO - 10.1091/mbc.E02-08-0493

M3 - Article

C2 - 12589041

AN - SCOPUS:0037328762

VL - 14

SP - 384

EP - 395

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

SN - 1059-1524

IS - 2

ER -