Determination of tadalafil and N-desmethylsibutramine in health and dietary supplements using ultra-performance liquid chromatography (UPLC) coupled with quadrupole-time-of-flight mass spectrometry (Q-TOF MS)

S. H. Roh, Y. P. Kang, S. Park, Y. Huh, J. Lee, J. H. Park, D. Kim, S. W. Kwon

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The adulteration of dietary supplements with drugs is potentially dangerous for human health. In this study, a method was used to test simultaneously for the presence of three synthetic PDE-5 inhibitors (sildenafil, vardenafil and tadalafil), and sibutramine and its two major metabolites (N-desmethylsibutramine and N-didesmethylsibutramine) using ultra-performance liquid chromatography (UPLC) coupled with quadrupole-time-of-flight mass spectrometry (Q-TOF MS) in dietary supplements. This approach with UPLC/Q-TOF MS uses the high accurate mass of six compounds for identification and has a short run time. The recovery was from 87% to 113%; precision was less than 12.8%. The limit of detection and limit of quantification were from 0.4 to 2.0 μg kg -1 and from 1.3 to 6.0 μg kg -1, respectively. This method allows easy and fast analysis and detection of diverse adulterants.

Original languageEnglish (US)
Pages (from-to)1475-1482
Number of pages8
JournalFood Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment
Volume28
Issue number11
DOIs
StatePublished - Nov 2011

Fingerprint

sibutramine
Dietary supplements
ultra-performance liquid chromatography
adulterated products
Liquid chromatography
Dietary Supplements
Liquid Chromatography
Mass spectrometry
dietary supplements
Mass Spectrometry
Health
mass spectrometry
Phosphodiesterase 5 Inhibitors
Metabolites
human health
Limit of Detection
detection limit
metabolites
Recovery
drugs

Keywords

  • additives
  • dietary supplements

ASJC Scopus subject areas

  • Food Science
  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health
  • Toxicology
  • Chemistry(all)

Cite this

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title = "Determination of tadalafil and N-desmethylsibutramine in health and dietary supplements using ultra-performance liquid chromatography (UPLC) coupled with quadrupole-time-of-flight mass spectrometry (Q-TOF MS)",
abstract = "The adulteration of dietary supplements with drugs is potentially dangerous for human health. In this study, a method was used to test simultaneously for the presence of three synthetic PDE-5 inhibitors (sildenafil, vardenafil and tadalafil), and sibutramine and its two major metabolites (N-desmethylsibutramine and N-didesmethylsibutramine) using ultra-performance liquid chromatography (UPLC) coupled with quadrupole-time-of-flight mass spectrometry (Q-TOF MS) in dietary supplements. This approach with UPLC/Q-TOF MS uses the high accurate mass of six compounds for identification and has a short run time. The recovery was from 87{\%} to 113{\%}; precision was less than 12.8{\%}. The limit of detection and limit of quantification were from 0.4 to 2.0 μg kg -1 and from 1.3 to 6.0 μg kg -1, respectively. This method allows easy and fast analysis and detection of diverse adulterants.",
keywords = "additives, dietary supplements",
author = "Roh, {S. H.} and Kang, {Y. P.} and S. Park and Y. Huh and J. Lee and Park, {J. H.} and D. Kim and Kwon, {S. W.}",
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AU - Roh, S. H.

AU - Kang, Y. P.

AU - Park, S.

AU - Huh, Y.

AU - Lee, J.

AU - Park, J. H.

AU - Kim, D.

AU - Kwon, S. W.

PY - 2011/11

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N2 - The adulteration of dietary supplements with drugs is potentially dangerous for human health. In this study, a method was used to test simultaneously for the presence of three synthetic PDE-5 inhibitors (sildenafil, vardenafil and tadalafil), and sibutramine and its two major metabolites (N-desmethylsibutramine and N-didesmethylsibutramine) using ultra-performance liquid chromatography (UPLC) coupled with quadrupole-time-of-flight mass spectrometry (Q-TOF MS) in dietary supplements. This approach with UPLC/Q-TOF MS uses the high accurate mass of six compounds for identification and has a short run time. The recovery was from 87% to 113%; precision was less than 12.8%. The limit of detection and limit of quantification were from 0.4 to 2.0 μg kg -1 and from 1.3 to 6.0 μg kg -1, respectively. This method allows easy and fast analysis and detection of diverse adulterants.

AB - The adulteration of dietary supplements with drugs is potentially dangerous for human health. In this study, a method was used to test simultaneously for the presence of three synthetic PDE-5 inhibitors (sildenafil, vardenafil and tadalafil), and sibutramine and its two major metabolites (N-desmethylsibutramine and N-didesmethylsibutramine) using ultra-performance liquid chromatography (UPLC) coupled with quadrupole-time-of-flight mass spectrometry (Q-TOF MS) in dietary supplements. This approach with UPLC/Q-TOF MS uses the high accurate mass of six compounds for identification and has a short run time. The recovery was from 87% to 113%; precision was less than 12.8%. The limit of detection and limit of quantification were from 0.4 to 2.0 μg kg -1 and from 1.3 to 6.0 μg kg -1, respectively. This method allows easy and fast analysis and detection of diverse adulterants.

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