Determination of tadalafil and N-desmethylsibutramine in health and dietary supplements using ultra-performance liquid chromatography (UPLC) coupled with quadrupole-time-of-flight mass spectrometry (Q-TOF MS)

S. H. Roh, Y. P. Kang, S. Park, Y. Huh, J. Lee, J. H. Park, D. Kim, S. W. Kwon

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The adulteration of dietary supplements with drugs is potentially dangerous for human health. In this study, a method was used to test simultaneously for the presence of three synthetic PDE-5 inhibitors (sildenafil, vardenafil and tadalafil), and sibutramine and its two major metabolites (N-desmethylsibutramine and N-didesmethylsibutramine) using ultra-performance liquid chromatography (UPLC) coupled with quadrupole-time-of-flight mass spectrometry (Q-TOF MS) in dietary supplements. This approach with UPLC/Q-TOF MS uses the high accurate mass of six compounds for identification and has a short run time. The recovery was from 87% to 113%; precision was less than 12.8%. The limit of detection and limit of quantification were from 0.4 to 2.0 μg kg -1 and from 1.3 to 6.0 μg kg -1, respectively. This method allows easy and fast analysis and detection of diverse adulterants.

Original languageEnglish (US)
Pages (from-to)1475-1482
Number of pages8
JournalFood Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment
Volume28
Issue number11
DOIs
StatePublished - Nov 2011

Keywords

  • additives
  • dietary supplements

ASJC Scopus subject areas

  • Food Science
  • Chemistry(all)
  • Toxicology
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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