Development and cross-species amplification of 18 microsatellite markers in the Sumatran tiger (Panthera tigris sumatrae)

J. E. Williamson, R. M. Huebinger, J. A. Sommer, E. E. Louis, R. C. Barber

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Eighteen polymorphic microsatellite loci from the highly endangered Sumatran tiger (Panthera tigris sumatrae) were isolated and characterized. Upon polymerase chain reaction amplification, 16 of these markers produced a single, sharp band in all three tiger and 10 non-tiger felid species examined. Of the two remaining loci, 6HDZ057 and 6HDZ635 failed to amplify genomic DNA from puma (Felis concolor) and cheetah (Acinonyx jubatus), respectively. The amplification of these markers across four genera is an indication of their usefulness for population genetics studies and conservation work in a wide range of felid species.

Original languageEnglish (US)
Pages (from-to)110-112
Number of pages3
JournalMolecular Ecology Notes
Volume2
Issue number2
DOIs
StatePublished - Jun 26 2002

Keywords

  • Cross-amplification
  • Felidae
  • Genetic markers
  • Microsatellites
  • Tiger

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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