TY - JOUR
T1 - Development of an assay for ozone-specific antioxidant capacity
AU - Rutkowski, Joseph M.
AU - Santiago, Lizzie Y.
AU - Ben-Jebria, Abdellaziz
AU - Ultman, James S.
N1 - Funding Information:
Received 7 April 2003; sent for revision 8 May 2003; accepted 16 June 2003. This research was supported by grant ES06075 from the National Institute of Environmental Health Sciences at the National Institutes of Health. A. Ben-Jebria is also affiliated with Institut National de la Santé et de la Recherche Medicale (INSERM), France. Address correspondence to James S. Ultman, PhD, Department of Chemical Engineering, Pennsylvania State University, 106 Fenske Laboratory, University Park, PA 16802, USA. E-mail: jsu@psu.edu
PY - 2003/11
Y1 - 2003/11
N2 - A method of determining the ozone-specific antioxidant capacity (OZAC) of lavage samples from the respiratory system was developed. Gaseous ozone (O 3) was produced in cuvettes by irradiation with an ultraviolet lamp; aliquots of sample or of a saline control were then added and sufficient time was allowed for ozonation to reach completion; and an aliquot of indigo trisulfonate (ITS) was added to react with excess O3. Because each molecule of O3, rapidly bleaches one molecule of the deeply colored ITS, an OZAC value in concentration units was computed from the difference in light absorbance between the sample and the saline control multiplied by the extinction coefficient of ITS. Experiments in 0-40 μM antioxidant solutions indicated that the OZAC values of uric acid and ascorbic acid were close to their actual concentrations and were independent of O3 concentration. On the other hand, the OZAC of reduced glutathione and possibly human nasal lavage were nonlinearly related to antioxidant concentration and were directly related to O3 concentration.
AB - A method of determining the ozone-specific antioxidant capacity (OZAC) of lavage samples from the respiratory system was developed. Gaseous ozone (O 3) was produced in cuvettes by irradiation with an ultraviolet lamp; aliquots of sample or of a saline control were then added and sufficient time was allowed for ozonation to reach completion; and an aliquot of indigo trisulfonate (ITS) was added to react with excess O3. Because each molecule of O3, rapidly bleaches one molecule of the deeply colored ITS, an OZAC value in concentration units was computed from the difference in light absorbance between the sample and the saline control multiplied by the extinction coefficient of ITS. Experiments in 0-40 μM antioxidant solutions indicated that the OZAC values of uric acid and ascorbic acid were close to their actual concentrations and were independent of O3 concentration. On the other hand, the OZAC of reduced glutathione and possibly human nasal lavage were nonlinearly related to antioxidant concentration and were directly related to O3 concentration.
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U2 - 10.1080/08958370390241876
DO - 10.1080/08958370390241876
M3 - Article
C2 - 14569498
AN - SCOPUS:0344009729
SN - 0895-8378
VL - 15
SP - 1369
EP - 1385
JO - Inhalation Toxicology
JF - Inhalation Toxicology
IS - 13
ER -