Developmental changes in the subcellular distribution of the 43K (v1) polypeptides in Torpedo marmorata electrocyte: Support for a role in acetylcholine receptor stabilization

Nghiêm Hoàng-Oanh, Joseph A Hill, Jean Pierre Changeux

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Analysis of the relative amounts of the acetylcholine receptors (AChR) and of the 43K protein present in the membrane of developing electrocyte shows that massive accumulation of 43K protein is not required for induction of early AChR clustering. Furthermore, we demonstrate the existence of cytosol- and membrane- associated 43K polypeptide pools in Torpedo electrocyte. Epitope analysis shows that both pools of 43K protein are related to the long mRNA transcript and share similar antigenic determinants distributed throughout the protein sequence. Their partition between the cytosol and membrane fractions abruptly increases in favor of the membrane during the postsynaptic maturation phase of development, supporting a role for 43K protein in the stabilization and maintenance of the postsynaptic domain.

Original languageEnglish (US)
Pages (from-to)1059-1067
Number of pages9
JournalDevelopment
Volume113
Issue number4
StatePublished - Dec 1991

Fingerprint

Torpedo
Cholinergic Receptors
Peptides
Membranes
Proteins
Cytosol
Epitopes
Cluster Analysis
Maintenance
Messenger RNA

Keywords

  • 43K
  • AChR clustering
  • Development
  • Synapse stabilization

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Developmental changes in the subcellular distribution of the 43K (v1) polypeptides in Torpedo marmorata electrocyte : Support for a role in acetylcholine receptor stabilization. / Hoàng-Oanh, Nghiêm; Hill, Joseph A; Changeux, Jean Pierre.

In: Development, Vol. 113, No. 4, 12.1991, p. 1059-1067.

Research output: Contribution to journalArticle

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