Diagnostics and laboratory role in outbreaks

Research output: Contribution to journalReview article

Abstract

Purpose of review: The review describes the investigative benefits of traditional and novel molecular epidemiology techniques, while acknowledging the limitations faced by clinical laboratories seeking to implement these methods. Recent findings: Pulse-field gel electrophoresis and other traditional techniques remain powerful tools in outbreak investigations and continue to be used by multiple groups. Newer techniques such as matrix-assisted laser desorption/ionization-time of flight mass-spectrometry and whole genome sequencing show great promise. However, there is a lack of standardization regarding definitions for genetic relatedness, nor are there established criteria for accuracy and reproducibility. There are also challenges regarding availability of trained bioinformatics staff, and concerns regarding reimbursement. Summary: There are many tools available for molecular epidemiologic investigation. Epidemiologists and clinical laboratorians should work together to determine which testing methods are best for each institution.

Original languageEnglish (US)
Pages (from-to)419-424
Number of pages6
JournalCurrent Opinion in Infectious Diseases
Volume30
Issue number4
DOIs
StatePublished - Aug 1 2017

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Disease Outbreaks
Molecular Epidemiology
Computational Biology
Electrophoresis
Mass Spectrometry
Lasers
Gels
Genome
Epidemiologists

Keywords

  • implementation challenges
  • molecular epidemiology
  • outbreak investigation
  • pulse-field gel electrophoresis
  • whole genome sequencing

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Diagnostics and laboratory role in outbreaks. / Lee, Francesca.

In: Current Opinion in Infectious Diseases, Vol. 30, No. 4, 01.08.2017, p. 419-424.

Research output: Contribution to journalReview article

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