Diaphanous regulates SCAR complex localization during Drosophila myoblast fusion

Su Deng, Ingo Bothe, Mary Baylies

Research output: Contribution to journalComment/debatepeer-review

Abstract

From Drosophila to man, multinucleated muscle cells form through cell-cell fusion. Using Drosophila as a model system, researchers first identified, and then demonstrated, the importance of actin cytoskeletal rearrangements at the site of fusion. These actin rearrangements at the fusion site are regulated by SCAR and WASp mediated Arp2/3 activation, which nucleates branched actin networks. Loss of SCAR, WASp or both leads to defects in myoblast fusion. Recently, we have found that the actin regulator Diaphanous (Dia) also plays a role both in organizing actin and in regulating Arp2/3 activity at the fusion site. In this Extra View article, we provide additional data showing that the Abi-SCAR complex accumulates at the fusion site and that excessive SCAR activity impairs myoblast fusion. Using constitutively active Dia constructs, we provide additional evidence that Dia functions upstream of SCAR activity to regulate actin dynamics at the fusion site and to localize the Abi-SCAR complex.

Original languageEnglish (US)
Pages (from-to)178-186
Number of pages9
JournalFly
Volume10
Issue number4
DOIs
StatePublished - Oct 1 2016
Externally publishedYes

Keywords

  • Abi
  • actin
  • Arp2/3
  • Diaphanous
  • myoblast fusion
  • SCAR/WAVE

ASJC Scopus subject areas

  • Insect Science

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