Dietary n-3 polyunsaturated fatty acids fail to reduce prostate tumorigenesis in the PB-ErbB-2 x Pten+/- preclinical mouse model

Sarada Vissapragada, Anup Ghosh, Lymor Ringer, Patricia Salinas, Amanda Brophy, Daniel Peaceman, Bhaskar Kallakury, Partha P. Banerjee, Stanley T. Fricke, William Helfrich, Yi Chien Lee, Richard Pestell, Philipp Scherer, Herbert B. Tanowitz, Maria Laura Avantaggiati, Leena Hilakivi-Clarke, Michael P. Lisanti, Olga C. Rodriguez, Chris Albanese

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Diet and obesity, and their associated metabolic alterations, are some of the fastest-growing causes of disease and death in America. Findings from epidemiological studies correlating obesity, the sources of dietary fat and prostate cancer (pCa) are conflicting. We have previously shown that 15% of pB-erbB-2 x pten+/- mice developed pCa and exhibited increased phosphorylated 4e-Bp1, but not the key PI3-kinase intermediary phospho-protein, mTOR, when maintained on unrefined mouse chow. We report herein that 100% of animals fed refined, westernized AIN-93-based diets containing corn oil developed PCa by 12 months of age. Increases in visceral fat and mTOR activation in the tumors were also observed. Furthermore, nuclear cyclin E levels were significantly induced by the AIN-93-corn oil-based diets versus chow. Replacing 50% of the corn oil with menhaden oil, with 21% of its triglycerides being n-3 PUFA's, had no effect on tumorigenesis, fat deposition, cyclin E or mTOR. Phosphorylated BAD levels were similar in the tumors of mice in all three diets. Our data demonstrated that in the context of our preclinical model, components of crude chow, but not dietary n-3 PUFAs, protect against PCa progression. In addition, these data establish phosphorylated mTOR, nuclear cyclin E and visceral fat deposits as possible biomarkers of increased dietary risk for PCa.

Original languageEnglish (US)
Pages (from-to)1824-1829
Number of pages6
JournalCell Cycle
Volume9
Issue number9
DOIs
StatePublished - May 1 2010

Fingerprint

Omega-3 Fatty Acids
Cyclin E
Corn Oil
Prostate
Carcinogenesis
Diet
Intra-Abdominal Fat
Prostatic Neoplasms
Obesity
TOR Serine-Threonine Kinases
Dietary Fats
Phosphatidylinositol 3-Kinases
Epidemiologic Studies
Cause of Death
Neoplasms
Triglycerides
Biomarkers
Fats

Keywords

  • Cyclin E
  • ErbB-2
  • MRI
  • mTOR
  • Polyunsaturated fatty acid
  • Prostate
  • Pten

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Cite this

Vissapragada, S., Ghosh, A., Ringer, L., Salinas, P., Brophy, A., Peaceman, D., ... Albanese, C. (2010). Dietary n-3 polyunsaturated fatty acids fail to reduce prostate tumorigenesis in the PB-ErbB-2 x Pten+/- preclinical mouse model. Cell Cycle, 9(9), 1824-1829. https://doi.org/10.4161/cc.9.9.11542

Dietary n-3 polyunsaturated fatty acids fail to reduce prostate tumorigenesis in the PB-ErbB-2 x Pten+/- preclinical mouse model. / Vissapragada, Sarada; Ghosh, Anup; Ringer, Lymor; Salinas, Patricia; Brophy, Amanda; Peaceman, Daniel; Kallakury, Bhaskar; Banerjee, Partha P.; Fricke, Stanley T.; Helfrich, William; Lee, Yi Chien; Pestell, Richard; Scherer, Philipp; Tanowitz, Herbert B.; Avantaggiati, Maria Laura; Hilakivi-Clarke, Leena; Lisanti, Michael P.; Rodriguez, Olga C.; Albanese, Chris.

In: Cell Cycle, Vol. 9, No. 9, 01.05.2010, p. 1824-1829.

Research output: Contribution to journalArticle

Vissapragada, S, Ghosh, A, Ringer, L, Salinas, P, Brophy, A, Peaceman, D, Kallakury, B, Banerjee, PP, Fricke, ST, Helfrich, W, Lee, YC, Pestell, R, Scherer, P, Tanowitz, HB, Avantaggiati, ML, Hilakivi-Clarke, L, Lisanti, MP, Rodriguez, OC & Albanese, C 2010, 'Dietary n-3 polyunsaturated fatty acids fail to reduce prostate tumorigenesis in the PB-ErbB-2 x Pten+/- preclinical mouse model', Cell Cycle, vol. 9, no. 9, pp. 1824-1829. https://doi.org/10.4161/cc.9.9.11542
Vissapragada, Sarada ; Ghosh, Anup ; Ringer, Lymor ; Salinas, Patricia ; Brophy, Amanda ; Peaceman, Daniel ; Kallakury, Bhaskar ; Banerjee, Partha P. ; Fricke, Stanley T. ; Helfrich, William ; Lee, Yi Chien ; Pestell, Richard ; Scherer, Philipp ; Tanowitz, Herbert B. ; Avantaggiati, Maria Laura ; Hilakivi-Clarke, Leena ; Lisanti, Michael P. ; Rodriguez, Olga C. ; Albanese, Chris. / Dietary n-3 polyunsaturated fatty acids fail to reduce prostate tumorigenesis in the PB-ErbB-2 x Pten+/- preclinical mouse model. In: Cell Cycle. 2010 ; Vol. 9, No. 9. pp. 1824-1829.
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