Differential alternative splicing of human transglutaminase 4 in benign prostate hyperplasia and prostate cancer

Sung Yup Cho, Kyungho Choi, Ju Hong Jeon, Chai Wan Kim, Dong Myung Shin, Jong Bouk Lee, Sang Eun Lee, Choung Soo Kim, Jeong Soo Park, Eui Man Jeong, Gi Yong Jang, Kye Yong Song, In Gyu Kim

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Transglutaminase 4 is a member of enzyme family that catalyzes calcium-dependent posttranslational modification of proteins. Although transglutaminase 4 has been shown to have prostate-restricted expression pattern, little is known about the biological function of transglutaminase 4 in human. To gain insight into its role in prostate, we analyzed the expression status of human transglutaminase 4 in benign prostate hyperplasia (BPH) and prostate cancer (PCa). Unexpectedly, RT-PCR and nucleotide sequence analysis showed four alternative splicing variants of transglutaminase 4: transglutaminase 4-L, -M (-M1 and -M2) and -S. The difference between transglutaminase 4-M1 and -M2 is attributed to splicing sites, but not nucleotide size. The deduced amino acid sequences showed that transglutaminase 4-L, -M1 and -M2 have correct open reading frames, whereas transglutaminase 4-S has a truncated reading frame. RT-PCR analysis of clinical samples revealed that transglutaminase 4-M and -S were detected in all tested prostate tissue (80 BPH and 48 PCa). Interestingly, transglutaminase 4-L was found in 56% of BPH (45 out of 80) and only in 15% of PCa (7 out of 48). However, transglutaminase 4-L expression did not correlate with serum prostate-specific antigen (PSA) level, prostate volumes or PSA densities. These results will provide a clue to future investigation aiming at delineating physiological and pathological roles of human transglutaminase 4.

Original languageEnglish (US)
Pages (from-to)310-318
Number of pages9
JournalExperimental and Molecular Medicine
Volume42
Issue number4
DOIs
StatePublished - 2010

Fingerprint

Alternative Splicing
Hyperplasia
Prostate
Prostatic Neoplasms
Prostate-Specific Antigen
transglutaminase 4
Nucleotides
Reading Frames
Polymerase Chain Reaction
Post Translational Protein Processing
Open Reading Frames
Sequence Analysis
Amino Acid Sequence

Keywords

  • Alternative splicing
  • Prostate hyperplasia
  • Prostatic neoplasms
  • Transglutaminase 4

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Molecular Medicine
  • Clinical Biochemistry
  • Medicine(all)

Cite this

Differential alternative splicing of human transglutaminase 4 in benign prostate hyperplasia and prostate cancer. / Cho, Sung Yup; Choi, Kyungho; Jeon, Ju Hong; Kim, Chai Wan; Shin, Dong Myung; Lee, Jong Bouk; Lee, Sang Eun; Kim, Choung Soo; Park, Jeong Soo; Jeong, Eui Man; Jang, Gi Yong; Song, Kye Yong; Kim, In Gyu.

In: Experimental and Molecular Medicine, Vol. 42, No. 4, 2010, p. 310-318.

Research output: Contribution to journalArticle

Cho, SY, Choi, K, Jeon, JH, Kim, CW, Shin, DM, Lee, JB, Lee, SE, Kim, CS, Park, JS, Jeong, EM, Jang, GY, Song, KY & Kim, IG 2010, 'Differential alternative splicing of human transglutaminase 4 in benign prostate hyperplasia and prostate cancer', Experimental and Molecular Medicine, vol. 42, no. 4, pp. 310-318. https://doi.org/10.3858/emm.2010.42.4.031
Cho, Sung Yup ; Choi, Kyungho ; Jeon, Ju Hong ; Kim, Chai Wan ; Shin, Dong Myung ; Lee, Jong Bouk ; Lee, Sang Eun ; Kim, Choung Soo ; Park, Jeong Soo ; Jeong, Eui Man ; Jang, Gi Yong ; Song, Kye Yong ; Kim, In Gyu. / Differential alternative splicing of human transglutaminase 4 in benign prostate hyperplasia and prostate cancer. In: Experimental and Molecular Medicine. 2010 ; Vol. 42, No. 4. pp. 310-318.
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AU - Shin, Dong Myung

AU - Lee, Jong Bouk

AU - Lee, Sang Eun

AU - Kim, Choung Soo

AU - Park, Jeong Soo

AU - Jeong, Eui Man

AU - Jang, Gi Yong

AU - Song, Kye Yong

AU - Kim, In Gyu

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