Differential alternative splicing of human transglutaminase 4 in benign prostate hyperplasia and prostate cancer

Sung Yup Cho, Kyungho Choi, Ju Hong Jeon, Chai Wan Kim, Dong Myung Shin, Jong Bouk Lee, Sang Eun Lee, Choung Soo Kim, Jeong Soo Park, Eui Man Jeong, Gi Yong Jang, Kye Yong Song, In Gyu Kim

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23 Scopus citations

Abstract

Transglutaminase 4 is a member of enzyme family that catalyzes calcium-dependent posttranslational modification of proteins. Although transglutaminase 4 has been shown to have prostate-restricted expression pattern, little is known about the biological function of transglutaminase 4 in human. To gain insight into its role in prostate, we analyzed the expression status of human transglutaminase 4 in benign prostate hyperplasia (BPH) and prostate cancer (PCa). Unexpectedly, RT-PCR and nucleotide sequence analysis showed four alternative splicing variants of transglutaminase 4: transglutaminase 4-L, -M (-M1 and -M2) and -S. The difference between transglutaminase 4-M1 and -M2 is attributed to splicing sites, but not nucleotide size. The deduced amino acid sequences showed that transglutaminase 4-L, -M1 and -M2 have correct open reading frames, whereas transglutaminase 4-S has a truncated reading frame. RT-PCR analysis of clinical samples revealed that transglutaminase 4-M and -S were detected in all tested prostate tissue (80 BPH and 48 PCa). Interestingly, transglutaminase 4-L was found in 56% of BPH (45 out of 80) and only in 15% of PCa (7 out of 48). However, transglutaminase 4-L expression did not correlate with serum prostate-specific antigen (PSA) level, prostate volumes or PSA densities. These results will provide a clue to future investigation aiming at delineating physiological and pathological roles of human transglutaminase 4.

Original languageEnglish (US)
Pages (from-to)310-318
Number of pages9
JournalExperimental and Molecular Medicine
Volume42
Issue number4
DOIs
StatePublished - Apr 30 2010

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Keywords

  • Alternative splicing
  • Prostate hyperplasia
  • Prostatic neoplasms
  • Transglutaminase 4

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Cho, S. Y., Choi, K., Jeon, J. H., Kim, C. W., Shin, D. M., Lee, J. B., Lee, S. E., Kim, C. S., Park, J. S., Jeong, E. M., Jang, G. Y., Song, K. Y., & Kim, I. G. (2010). Differential alternative splicing of human transglutaminase 4 in benign prostate hyperplasia and prostate cancer. Experimental and Molecular Medicine, 42(4), 310-318. https://doi.org/10.3858/emm.2010.42.4.031