Differential effects of light and feeding on circadian organization of peripheral clocks in a forebrain Bmal1 mutant

Mariko Izumo, Martina Pejchal, Andrew C. Schook, Ryan P. Lange, Jacqueline A. Walisser, Takashi R. Sato, Xiaozhong Wang, Christopher A. Bradfield, Joseph S. Takahashi

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

In order to assess the contribution of a central clock in the hypothalamic suprachiasmatic nucleus (SCN) to circadian behavior and the organization of peripheral clocks, we generated forebrain/SCN-specific Bmal1 knockout mice by using floxed Bmal1 and pan-neuronal Cre lines. The forebrain knockout mice showed >90% deletion of BMAL1 in the SCN and exhibited an immediate and complete loss of circadian behavior in constant conditions. Circadian rhythms in peripheral tissues persisted but became desynchronized and damped in constant darkness. The loss of synchrony was rescued by light/dark cycles and partially by restricted feeding (only in the liver and kidney but not in the other tissues) in a distinct manner. These results suggest that the forebrain/SCN is essential for internal temporal order of robust circadian programs in peripheral clocks, and that individual peripheral clocks are affected differently by light and feeding in the absence of a functional oscillator in the forebrain.

Original languageEnglish (US)
JournaleLife
Volume3
DOIs
StatePublished - 2014

Fingerprint

Suprachiasmatic Nucleus
Prosencephalon
Clocks
Light
Knockout Mice
Tissue
Darkness
Photoperiod
Circadian Rhythm
Liver
Kidney

Keywords

  • Arntl
  • Bmal1
  • circadian rhythms
  • Cre/loxP
  • light entrainment
  • Mop3
  • mouse
  • neuroscience
  • peripheral clocks
  • suprachiasmatic nucleus

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Differential effects of light and feeding on circadian organization of peripheral clocks in a forebrain Bmal1 mutant. / Izumo, Mariko; Pejchal, Martina; Schook, Andrew C.; Lange, Ryan P.; Walisser, Jacqueline A.; Sato, Takashi R.; Wang, Xiaozhong; Bradfield, Christopher A.; Takahashi, Joseph S.

In: eLife, Vol. 3, 2014.

Research output: Contribution to journalArticle

Izumo, Mariko ; Pejchal, Martina ; Schook, Andrew C. ; Lange, Ryan P. ; Walisser, Jacqueline A. ; Sato, Takashi R. ; Wang, Xiaozhong ; Bradfield, Christopher A. ; Takahashi, Joseph S. / Differential effects of light and feeding on circadian organization of peripheral clocks in a forebrain Bmal1 mutant. In: eLife. 2014 ; Vol. 3.
@article{52e8b4c7ee0f4b8c9d7957ffcad1bb48,
title = "Differential effects of light and feeding on circadian organization of peripheral clocks in a forebrain Bmal1 mutant",
abstract = "In order to assess the contribution of a central clock in the hypothalamic suprachiasmatic nucleus (SCN) to circadian behavior and the organization of peripheral clocks, we generated forebrain/SCN-specific Bmal1 knockout mice by using floxed Bmal1 and pan-neuronal Cre lines. The forebrain knockout mice showed >90{\%} deletion of BMAL1 in the SCN and exhibited an immediate and complete loss of circadian behavior in constant conditions. Circadian rhythms in peripheral tissues persisted but became desynchronized and damped in constant darkness. The loss of synchrony was rescued by light/dark cycles and partially by restricted feeding (only in the liver and kidney but not in the other tissues) in a distinct manner. These results suggest that the forebrain/SCN is essential for internal temporal order of robust circadian programs in peripheral clocks, and that individual peripheral clocks are affected differently by light and feeding in the absence of a functional oscillator in the forebrain.",
keywords = "Arntl, Bmal1, circadian rhythms, Cre/loxP, light entrainment, Mop3, mouse, neuroscience, peripheral clocks, suprachiasmatic nucleus",
author = "Mariko Izumo and Martina Pejchal and Schook, {Andrew C.} and Lange, {Ryan P.} and Walisser, {Jacqueline A.} and Sato, {Takashi R.} and Xiaozhong Wang and Bradfield, {Christopher A.} and Takahashi, {Joseph S.}",
year = "2014",
doi = "10.7554/eLife.04617",
language = "English (US)",
volume = "3",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",

}

TY - JOUR

T1 - Differential effects of light and feeding on circadian organization of peripheral clocks in a forebrain Bmal1 mutant

AU - Izumo, Mariko

AU - Pejchal, Martina

AU - Schook, Andrew C.

AU - Lange, Ryan P.

AU - Walisser, Jacqueline A.

AU - Sato, Takashi R.

AU - Wang, Xiaozhong

AU - Bradfield, Christopher A.

AU - Takahashi, Joseph S.

PY - 2014

Y1 - 2014

N2 - In order to assess the contribution of a central clock in the hypothalamic suprachiasmatic nucleus (SCN) to circadian behavior and the organization of peripheral clocks, we generated forebrain/SCN-specific Bmal1 knockout mice by using floxed Bmal1 and pan-neuronal Cre lines. The forebrain knockout mice showed >90% deletion of BMAL1 in the SCN and exhibited an immediate and complete loss of circadian behavior in constant conditions. Circadian rhythms in peripheral tissues persisted but became desynchronized and damped in constant darkness. The loss of synchrony was rescued by light/dark cycles and partially by restricted feeding (only in the liver and kidney but not in the other tissues) in a distinct manner. These results suggest that the forebrain/SCN is essential for internal temporal order of robust circadian programs in peripheral clocks, and that individual peripheral clocks are affected differently by light and feeding in the absence of a functional oscillator in the forebrain.

AB - In order to assess the contribution of a central clock in the hypothalamic suprachiasmatic nucleus (SCN) to circadian behavior and the organization of peripheral clocks, we generated forebrain/SCN-specific Bmal1 knockout mice by using floxed Bmal1 and pan-neuronal Cre lines. The forebrain knockout mice showed >90% deletion of BMAL1 in the SCN and exhibited an immediate and complete loss of circadian behavior in constant conditions. Circadian rhythms in peripheral tissues persisted but became desynchronized and damped in constant darkness. The loss of synchrony was rescued by light/dark cycles and partially by restricted feeding (only in the liver and kidney but not in the other tissues) in a distinct manner. These results suggest that the forebrain/SCN is essential for internal temporal order of robust circadian programs in peripheral clocks, and that individual peripheral clocks are affected differently by light and feeding in the absence of a functional oscillator in the forebrain.

KW - Arntl

KW - Bmal1

KW - circadian rhythms

KW - Cre/loxP

KW - light entrainment

KW - Mop3

KW - mouse

KW - neuroscience

KW - peripheral clocks

KW - suprachiasmatic nucleus

UR - http://www.scopus.com/inward/record.url?scp=85003265692&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85003265692&partnerID=8YFLogxK

U2 - 10.7554/eLife.04617

DO - 10.7554/eLife.04617

M3 - Article

VL - 3

JO - eLife

JF - eLife

SN - 2050-084X

ER -