Differential expression of hepatocyte growth factor in liver kidney, lung, and spleen following burn in rats

Yasushi Yamashita, Marc G. Jeschke, Steven E. Wolf

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Hepatocyte growth factor (HGF) plays a role as an organotropic factor for regeneration of injured organs. HGF is synthesized as an inactive single-chain precursor which is then converted to a biologically active heterodimeric form by proteolytic processing. Burn is the insult that results in hypovolemia which causes systemic organ injury. In this study, we investigated the induction and activation of HGF in various rat organs following burn trauma. Tissue HGF content determined as the total amount of the single-chain and heterodimeric form increased significantly in liver, lung, spleen, and kidney 12 h after burn. Molecular analysis revealed that HGF in these four organs of control rats was the single-chain precursor. In the burned rats, HGF was the single-chain form in the liver and lung, whereas heterodimeric HGF was detected in the spleen and kidney. Tissue protein content, an index of tissue injury, descreased significantly in the spleen and kidney, indicating that tissue damage was severe in these two organs. These results suggest that burn induces the production of HGF in various organs, and that the induced HGF is activated according to the severity of tissue damage caused by burn. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)1293-1298
Number of pages6
JournalCytokine
Volume12
Issue number9
DOIs
StatePublished - Jan 1 2000

Keywords

  • Burn
  • Hepatocyte growth factor
  • Proteolytic activation
  • Rats

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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