Differential mitotic activation of endogenous c-Src, c-Yes, and Lyn in HeLa cells

Takahisa Kuga, Yuji Nakayama, Masaki Hoshino, Yukihiro Higashiyama, Yuuki Obata, Daisuke Matsuda, Kousuke Kasahara, Yasunori Fukumoto, Naoto Yamaguchi

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Src-family tyrosine kinases (SFKs) play an important role in mitosis. Despite overlapping expression of multiple SFK members, little is known about how individual SFK members are activated in M phase. Here, we examined mitotic activation of endogenous c-Src, c-Yes, and Lyn, which are co-expressed in HeLa cells. c-Src, c-Yes, and Lyn were activated at different levels in M phase, and the activation was inhibited by Cdc2 inactivation. Mitotic c-Src and c-Yes exhibited normal- and retarded-electrophoretic-mobility forms on SDS-polyacrylamide gels, whereas Lyn did not show mobility retardation. Like c-Src, the retardation of electrophoretic mobility of c-Yes was caused by Cdc2-mediated phosphorylation. The normal- and retarded-mobility forms of c-Src were comparably activated, but activation of the retarded-mobility form of c-Yes was higher than that of the normal-mobility form of c-Yes. Thus, these results suggest that endogenous c-Src, c-Yes, and Lyn are differentially activated through Cdc2 activation during M phase.

Original languageEnglish (US)
Pages (from-to)116-124
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume466
Issue number1
DOIs
StatePublished - Oct 1 2007
Externally publishedYes

Keywords

  • c-Src
  • c-Yes
  • Cdc2
  • Electrophoretic mobility
  • Fyn
  • Lyn
  • M phase
  • Mitosis
  • Phosphorylation
  • Src-family tyrosine kinase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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