Differentiating Proteomic Biomarkers in Breast Cancer by Laser Capture Microdissection and MALDI MS

Melinda E. Sanders, Eduardo C. Dias, Baogang J. Xu, James A. Mobley, Dean Billheimer, Heinrich Roder, Julia Grigorieva, Mitchell Dowsett, Carlos L. Arteaga, Richard M. Caprioli

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

We assessed proteomic patterns in breast cancer using MALDI MS and laser capture microdissected cells. Protein and peptide expression in invasive mammary carcinoma versus normal mammary epithelium and estrogen-receptor positive versus estrogen-receptor negative tumors were compared. Biomarker candidates were identified by statistical analysis and classifiers were developed and validated in blinded test sets. Several of the mlz features used in the classifiers were identified by LC-MS/MS and two were confirmed by immunohistochemistry.

Original languageEnglish (US)
Pages (from-to)1500-1507
Number of pages8
JournalJournal of Proteome Research
Volume7
Issue number4
DOIs
StatePublished - Apr 1 2008

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Keywords

  • Breast cancer
  • Laser capture microdissection
  • MALDI MS

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Sanders, M. E., Dias, E. C., Xu, B. J., Mobley, J. A., Billheimer, D., Roder, H., Grigorieva, J., Dowsett, M., Arteaga, C. L., & Caprioli, R. M. (2008). Differentiating Proteomic Biomarkers in Breast Cancer by Laser Capture Microdissection and MALDI MS. Journal of Proteome Research, 7(4), 1500-1507. https://doi.org/10.1021/pr7008109