Dimer-to-tetramer assembly of Lac repressor involves a leucine heptad repeat

S. Alberti, S. Oehler, B. Wilcken-Bergmann v., H. Kramer, B. Muller-Hill

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The C-terminus of Lac repressor is responsible for the formation of repressor tetramers from active dimers. If properly grafted, the C-terminus of Lac repressor (amino acids 331 to 360) converts Gal repressor dimers into tetramers. Amino acids 342 to 356 of Lac repressor contain a 4-3 hydrophobic repeat of four leucines and one valine. Systematic amino acid replacements of all residues in this region show that the protein-protein interaction between repressor dimers depends mainly on the hydrophobic residues of the 4-3 repeat, which is constitutive for coiled coils. Thus the tetramerization site of Lac repressor resembles the leucine zipper motif found in a family of eukaryotic transcription factors.

Original languageEnglish (US)
Pages (from-to)57-62
Number of pages6
JournalNew Biologist
Volume3
Issue number1
StatePublished - 1991

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Lac Repressors
Leucine
Dimers
Amino Acids
Leucine Zippers
Valine
Proteins
Transcription Factors

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Alberti, S., Oehler, S., Wilcken-Bergmann v., B., Kramer, H., & Muller-Hill, B. (1991). Dimer-to-tetramer assembly of Lac repressor involves a leucine heptad repeat. New Biologist, 3(1), 57-62.

Dimer-to-tetramer assembly of Lac repressor involves a leucine heptad repeat. / Alberti, S.; Oehler, S.; Wilcken-Bergmann v., B.; Kramer, H.; Muller-Hill, B.

In: New Biologist, Vol. 3, No. 1, 1991, p. 57-62.

Research output: Contribution to journalArticle

Alberti, S, Oehler, S, Wilcken-Bergmann v., B, Kramer, H & Muller-Hill, B 1991, 'Dimer-to-tetramer assembly of Lac repressor involves a leucine heptad repeat', New Biologist, vol. 3, no. 1, pp. 57-62.
Alberti S, Oehler S, Wilcken-Bergmann v. B, Kramer H, Muller-Hill B. Dimer-to-tetramer assembly of Lac repressor involves a leucine heptad repeat. New Biologist. 1991;3(1):57-62.
Alberti, S. ; Oehler, S. ; Wilcken-Bergmann v., B. ; Kramer, H. ; Muller-Hill, B. / Dimer-to-tetramer assembly of Lac repressor involves a leucine heptad repeat. In: New Biologist. 1991 ; Vol. 3, No. 1. pp. 57-62.
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