Dioxin catabolic genes are dispersed on the Terrabacter sp. DBF63 genome

Hideaki Nojiri; Mayuko Kamakura; Masaaki Urata; Takahiro Tanaka; Jin Sung Chung; Tetsuo Takemura; Takako Yoshida; Hiroshi Habe; Toshio Omori

doi:10.1016/S0006-291X(02)00873-2

Dioxin catabolic genes are dispersed on the Terrabacter sp. DBF63 genome

Hideaki Nojiri, Mayuko Kamakura, Masaaki Urata, Takahiro Tanaka, Jin Sung Chung, Tetsuo Takemura, Takako Yoshida, Hiroshi Habe, Toshio Omori

Dermatology

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Reverse transcription-PCR of the dbfA1A2, dbfBC, and pht genes, encoding oxygenase component of multicomponent dioxygenase, meta cleavage enzyme and hydrolase, and phthalate-degrading enzymes, respectively, revealed their role in the aromatic compound degradation by Terrabacter sp. strain DBF63. The specific expression in strain DBF63 cells grown on dibenzofuran (the model compound of dioxin; DF) and/or fluorene (FN) indicated that the DbfA1A2 and DbfBC catalyze the conversion of DF to salicylate, and that the DbfA1A2 and Pht enzymes are involved in FN degradation. Pulsed-field gel electrophoresis analyses revealed that the dbfA1A2 cistron and pht operon were located on the two linear plasmids, pDBF1 (160 kb) and pDBF2 (190 kb), while dbfBC genes were located on the chromosome. Because the pht operon is located immediately upstream of the dbfA1A2 cistron, the dioxin-catabolic genes were dispersed on the genome of strain DBF63, while FN-catabolic genes were gathered on the plasmids.

Original language	English (US)
Pages (from-to)	233-240
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	296
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(02)00873-2
State	Published - 2002

Keywords

Biodegradation
Degradative plasmid
Dibenzofuran
Dioxin
Expression
Fluorene
Terrabacter sp. strain DBF63
dbf Gene cluster
pDBF1

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(02)00873-2

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title = "Dioxin catabolic genes are dispersed on the Terrabacter sp. DBF63 genome",

abstract = "Reverse transcription-PCR of the dbfA1A2, dbfBC, and pht genes, encoding oxygenase component of multicomponent dioxygenase, meta cleavage enzyme and hydrolase, and phthalate-degrading enzymes, respectively, revealed their role in the aromatic compound degradation by Terrabacter sp. strain DBF63. The specific expression in strain DBF63 cells grown on dibenzofuran (the model compound of dioxin; DF) and/or fluorene (FN) indicated that the DbfA1A2 and DbfBC catalyze the conversion of DF to salicylate, and that the DbfA1A2 and Pht enzymes are involved in FN degradation. Pulsed-field gel electrophoresis analyses revealed that the dbfA1A2 cistron and pht operon were located on the two linear plasmids, pDBF1 (160 kb) and pDBF2 (190 kb), while dbfBC genes were located on the chromosome. Because the pht operon is located immediately upstream of the dbfA1A2 cistron, the dioxin-catabolic genes were dispersed on the genome of strain DBF63, while FN-catabolic genes were gathered on the plasmids.",

keywords = "Biodegradation, Degradative plasmid, Dibenzofuran, Dioxin, Expression, Fluorene, Terrabacter sp. strain DBF63, dbf Gene cluster, pDBF1",

author = "Hideaki Nojiri and Mayuko Kamakura and Masaaki Urata and Takahiro Tanaka and Chung, {Jin Sung} and Tetsuo Takemura and Takako Yoshida and Hiroshi Habe and Toshio Omori",

year = "2002",

doi = "10.1016/S0006-291X(02)00873-2",

language = "English (US)",

volume = "296",

pages = "233--240",

journal = "Biochemical and Biophysical Research Communications",

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publisher = "Academic Press Inc.",

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TY - JOUR

T1 - Dioxin catabolic genes are dispersed on the Terrabacter sp. DBF63 genome

AU - Nojiri, Hideaki

AU - Kamakura, Mayuko

AU - Urata, Masaaki

AU - Tanaka, Takahiro

AU - Chung, Jin Sung

AU - Takemura, Tetsuo

AU - Yoshida, Takako

AU - Habe, Hiroshi

AU - Omori, Toshio

PY - 2002

Y1 - 2002

N2 - Reverse transcription-PCR of the dbfA1A2, dbfBC, and pht genes, encoding oxygenase component of multicomponent dioxygenase, meta cleavage enzyme and hydrolase, and phthalate-degrading enzymes, respectively, revealed their role in the aromatic compound degradation by Terrabacter sp. strain DBF63. The specific expression in strain DBF63 cells grown on dibenzofuran (the model compound of dioxin; DF) and/or fluorene (FN) indicated that the DbfA1A2 and DbfBC catalyze the conversion of DF to salicylate, and that the DbfA1A2 and Pht enzymes are involved in FN degradation. Pulsed-field gel electrophoresis analyses revealed that the dbfA1A2 cistron and pht operon were located on the two linear plasmids, pDBF1 (160 kb) and pDBF2 (190 kb), while dbfBC genes were located on the chromosome. Because the pht operon is located immediately upstream of the dbfA1A2 cistron, the dioxin-catabolic genes were dispersed on the genome of strain DBF63, while FN-catabolic genes were gathered on the plasmids.

AB - Reverse transcription-PCR of the dbfA1A2, dbfBC, and pht genes, encoding oxygenase component of multicomponent dioxygenase, meta cleavage enzyme and hydrolase, and phthalate-degrading enzymes, respectively, revealed their role in the aromatic compound degradation by Terrabacter sp. strain DBF63. The specific expression in strain DBF63 cells grown on dibenzofuran (the model compound of dioxin; DF) and/or fluorene (FN) indicated that the DbfA1A2 and DbfBC catalyze the conversion of DF to salicylate, and that the DbfA1A2 and Pht enzymes are involved in FN degradation. Pulsed-field gel electrophoresis analyses revealed that the dbfA1A2 cistron and pht operon were located on the two linear plasmids, pDBF1 (160 kb) and pDBF2 (190 kb), while dbfBC genes were located on the chromosome. Because the pht operon is located immediately upstream of the dbfA1A2 cistron, the dioxin-catabolic genes were dispersed on the genome of strain DBF63, while FN-catabolic genes were gathered on the plasmids.

KW - Biodegradation

KW - Degradative plasmid

KW - Dibenzofuran

KW - Dioxin

KW - Expression

KW - Fluorene

KW - Terrabacter sp. strain DBF63

KW - dbf Gene cluster

KW - pDBF1

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SN - 0006-291X

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EP - 240

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Dioxin catabolic genes are dispersed on the Terrabacter sp. DBF63 genome

Abstract

Keywords

ASJC Scopus subject areas

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