Dioxin catabolic genes are dispersed on the Terrabacter sp. DBF63 genome

Hideaki Nojiri, Mayuko Kamakura, Masaaki Urata, Takahiro Tanaka, Jin Sung Chung, Tetsuo Takemura, Takako Yoshida, Hiroshi Habe, Toshio Omori

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Reverse transcription-PCR of the dbfA1A2, dbfBC, and pht genes, encoding oxygenase component of multicomponent dioxygenase, meta cleavage enzyme and hydrolase, and phthalate-degrading enzymes, respectively, revealed their role in the aromatic compound degradation by Terrabacter sp. strain DBF63. The specific expression in strain DBF63 cells grown on dibenzofuran (the model compound of dioxin; DF) and/or fluorene (FN) indicated that the DbfA1A2 and DbfBC catalyze the conversion of DF to salicylate, and that the DbfA1A2 and Pht enzymes are involved in FN degradation. Pulsed-field gel electrophoresis analyses revealed that the dbfA1A2 cistron and pht operon were located on the two linear plasmids, pDBF1 (160 kb) and pDBF2 (190 kb), while dbfBC genes were located on the chromosome. Because the pht operon is located immediately upstream of the dbfA1A2 cistron, the dioxin-catabolic genes were dispersed on the genome of strain DBF63, while FN-catabolic genes were gathered on the plasmids.

Original languageEnglish (US)
Pages (from-to)233-240
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume296
Issue number2
DOIs
StatePublished - Oct 14 2002

Keywords

  • Biodegradation
  • Degradative plasmid
  • Dibenzofuran
  • Dioxin
  • Expression
  • Fluorene
  • Terrabacter sp. strain DBF63
  • dbf Gene cluster
  • pDBF1

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Nojiri, H., Kamakura, M., Urata, M., Tanaka, T., Chung, J. S., Takemura, T., Yoshida, T., Habe, H., & Omori, T. (2002). Dioxin catabolic genes are dispersed on the Terrabacter sp. DBF63 genome. Biochemical and Biophysical Research Communications, 296(2), 233-240. https://doi.org/10.1016/S0006-291X(02)00873-2