Diphospho-myo-inositol phosphates from Dictyostelium identified as D-5-diphospho-myo-inositol pentakisphosphate and D-5,6-bisdiphospho-myo-inositol tetrakisphosphate

T. Laussmann, K. M. Reddy, K. K. Reddy, J. R. Falck, G. Vogel

Research output: Contribution to journalArticle

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Two diphospho-myo-inositol phosphates from Dictyostelium were recently investigated by two-dimensional 1H/31P NMR analysis and assigned to be either D-4-diphospho-myo-inositol pentakisphosphate (D-4-PP-InsP5) and D-4,5-bisdiphospho-myo-inositol tetrakisphosphate (D-4,5-bis-PP-InsP4) or their corresponding enantiomers D-6-PP-InsP5 and D-5,6-bis-PP-InsP4. In the present study the naturally occurring enantiomers were identified by using defined synthetic PP-InsP5 isomers as substrates for a partially purified PP-InsP5 5-kinase from Dictyostelium. This enzyme specifically phosphorylates the naturally occurring PP-InsP5 and the synthetic D-6-PP-InsP5, leading to D-5,6-bis-PP-InsP4. In contrast, neither D-4-PP-InsP5 norD-1-PP-InsP5 or D-3-PP-InsP5 are converted by the enzyme.

Original languageEnglish (US)
Pages (from-to)31-33
Number of pages3
JournalBiochemical Journal
Issue number1
Publication statusPublished - 1997


ASJC Scopus subject areas

  • Biochemistry

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