Disruption of the yeast N-myristoyl transferase gene causes recessive lethality

Robert J. Duronio, Dwight A. Towler, Robert O. Heuckeroth, Jeffrey I. Gordon

Research output: Contribution to journalArticlepeer-review

155 Scopus citations

Abstract

The structural gene for N-myristoyl transferase (NMT1) has been cloned from the budding yeast Saccharomyces cerevisiae. The gene encodes a polypeptide of 455 amino acids (Mr = 52,837) that has no identifiable significant primary sequence homology with any protein in currently available databases. Overexpression of NMT activity was achieved by means of the yeast episomal plasmid YEp24 without obvious effects on growth kinetics, cell morphology, or acylprotein metabolic labeling patterns. Insertional mutagenesis of the NMT1 locus on yeast chromosome XII caused recessive lethality, indicating that this protein acyltransferase activity is necessary for vegetative cell growth.

Original languageEnglish (US)
Pages (from-to)796-800
Number of pages5
JournalScience
Volume243
Issue number4892
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • General

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