Disruption of the yeast N-myristoyl transferase gene causes recessive lethality

Robert J. Duronio, Dwight A. Towler, Robert O. Heuckeroth, Jeffrey I. Gordon

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

The structural gene for N-myristoyl transferase (NMT1) has been cloned from the budding yeast Saccharomyces cerevisiae. The gene encodes a polypeptide of 455 amino acids (Mr = 52,837) that has no identifiable significant primary sequence homology with any protein in currently available databases. Overexpression of NMT activity was achieved by means of the yeast episomal plasmid YEp24 without obvious effects on growth kinetics, cell morphology, or acylprotein metabolic labeling patterns. Insertional mutagenesis of the NMT1 locus on yeast chromosome XII caused recessive lethality, indicating that this protein acyltransferase activity is necessary for vegetative cell growth.

Original languageEnglish (US)
Pages (from-to)796-800
Number of pages5
JournalScience
Volume243
Issue number4892
StatePublished - Jan 1 1989

Fingerprint

Recessive Genes
Transferases
Yeasts
Saccharomycetales
Insertional Mutagenesis
Growth
Sequence Homology
Genes
Saccharomyces cerevisiae
Plasmids
Chromosomes
Databases
Amino Acids
Peptides
Proteins
protein acyltransferase

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Duronio, R. J., Towler, D. A., Heuckeroth, R. O., & Gordon, J. I. (1989). Disruption of the yeast N-myristoyl transferase gene causes recessive lethality. Science, 243(4892), 796-800.

Disruption of the yeast N-myristoyl transferase gene causes recessive lethality. / Duronio, Robert J.; Towler, Dwight A.; Heuckeroth, Robert O.; Gordon, Jeffrey I.

In: Science, Vol. 243, No. 4892, 01.01.1989, p. 796-800.

Research output: Contribution to journalArticle

Duronio, RJ, Towler, DA, Heuckeroth, RO & Gordon, JI 1989, 'Disruption of the yeast N-myristoyl transferase gene causes recessive lethality', Science, vol. 243, no. 4892, pp. 796-800.
Duronio RJ, Towler DA, Heuckeroth RO, Gordon JI. Disruption of the yeast N-myristoyl transferase gene causes recessive lethality. Science. 1989 Jan 1;243(4892):796-800.
Duronio, Robert J. ; Towler, Dwight A. ; Heuckeroth, Robert O. ; Gordon, Jeffrey I. / Disruption of the yeast N-myristoyl transferase gene causes recessive lethality. In: Science. 1989 ; Vol. 243, No. 4892. pp. 796-800.
@article{709bc8484b0e4a0ca2e5fe65e2636b19,
title = "Disruption of the yeast N-myristoyl transferase gene causes recessive lethality",
abstract = "The structural gene for N-myristoyl transferase (NMT1) has been cloned from the budding yeast Saccharomyces cerevisiae. The gene encodes a polypeptide of 455 amino acids (Mr = 52,837) that has no identifiable significant primary sequence homology with any protein in currently available databases. Overexpression of NMT activity was achieved by means of the yeast episomal plasmid YEp24 without obvious effects on growth kinetics, cell morphology, or acylprotein metabolic labeling patterns. Insertional mutagenesis of the NMT1 locus on yeast chromosome XII caused recessive lethality, indicating that this protein acyltransferase activity is necessary for vegetative cell growth.",
author = "Duronio, {Robert J.} and Towler, {Dwight A.} and Heuckeroth, {Robert O.} and Gordon, {Jeffrey I.}",
year = "1989",
month = "1",
day = "1",
language = "English (US)",
volume = "243",
pages = "796--800",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "4892",

}

TY - JOUR

T1 - Disruption of the yeast N-myristoyl transferase gene causes recessive lethality

AU - Duronio, Robert J.

AU - Towler, Dwight A.

AU - Heuckeroth, Robert O.

AU - Gordon, Jeffrey I.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - The structural gene for N-myristoyl transferase (NMT1) has been cloned from the budding yeast Saccharomyces cerevisiae. The gene encodes a polypeptide of 455 amino acids (Mr = 52,837) that has no identifiable significant primary sequence homology with any protein in currently available databases. Overexpression of NMT activity was achieved by means of the yeast episomal plasmid YEp24 without obvious effects on growth kinetics, cell morphology, or acylprotein metabolic labeling patterns. Insertional mutagenesis of the NMT1 locus on yeast chromosome XII caused recessive lethality, indicating that this protein acyltransferase activity is necessary for vegetative cell growth.

AB - The structural gene for N-myristoyl transferase (NMT1) has been cloned from the budding yeast Saccharomyces cerevisiae. The gene encodes a polypeptide of 455 amino acids (Mr = 52,837) that has no identifiable significant primary sequence homology with any protein in currently available databases. Overexpression of NMT activity was achieved by means of the yeast episomal plasmid YEp24 without obvious effects on growth kinetics, cell morphology, or acylprotein metabolic labeling patterns. Insertional mutagenesis of the NMT1 locus on yeast chromosome XII caused recessive lethality, indicating that this protein acyltransferase activity is necessary for vegetative cell growth.

UR - http://www.scopus.com/inward/record.url?scp=0024590280&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024590280&partnerID=8YFLogxK

M3 - Article

VL - 243

SP - 796

EP - 800

JO - Science

JF - Science

SN - 0036-8075

IS - 4892

ER -