@article{050c7190737a44e29a9deb8d4c2c2d41,
title = "Dissecting mammalian spermatogenesis using spatial transcriptomics",
abstract = "Single-cell RNA sequencing has revealed extensive molecular diversity in gene programs governing mammalian spermatogenesis but fails to delineate their dynamics in the native context of seminiferous tubules, the spatially confined functional units of spermatogenesis. Here, we use Slide-seq, a spatial transcriptomics technology, to generate an atlas that captures the spatial gene expression patterns at near-single-cell resolution in the mouse and human testis. Using Slide-seq data, we devise a computational framework that accurately localizes testicular cell types in individual seminiferous tubules. Unbiased analysis systematically identifies spatially patterned genes and gene programs. Combining Slide-seq with targeted in situ RNA sequencing, we demonstrate significant differences in the cellular compositions of spermatogonial microenvironment between mouse and human testes. Finally, a comparison of the spatial atlas generated from the wild-type and diabetic mouse testis reveals a disruption in the spatial cellular organization of seminiferous tubules as a potential mechanism of diabetes-induced male infertility.",
author = "Haiqi Chen and Evan Murray and Anubhav Sinha and Anisha Laumas and Jilong Li and Daniel Lesman and Xichen Nie and Jim Hotaling and Jingtao Guo and Cairns, {Bradley R.} and Macosko, {Evan Z.} and Cheng, {C. Yan} and Fei Chen",
note = "Funding Information: We thank Jamie Marshall for help with mouse testicular sample collections and Paul Reginato for help with sample preparation for targeted in situ sequencing. We also thank Dylan Cable, Aleks Goeva, Sarah Mangiameli, Sophia Liu, and Yu Jiang for discussions on computational analysis and Tongtong Zhao for comments on the project. This work was supported by an NIH New Innovator Award ( DP2 AG058488-01 to E.Z.M.), an NIH Early Independence Award ( DP5, 1DP5OD024583 to F.C.), the NHGRI (grant R01, R01HG010647 to E.Z.M. and F.C.), the Burroughs Wellcome Fund CASI award (to F.C.), NICHD R01 HD056034 to C.Y.C., and the Schmidt Fellows Program at the Broad Institute and the Stanley Center for Psychiatric Research (F.C.). H.C. thanks the Lalor Foundation and the Male Contraceptive Initiative for their support. A.S. was supported by NIH/NIBIB MEMP Neuroimaging Training Program grant 2T32EB001680 . B.R.C. was supported by the Howard Hughes Medical Institute . Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
month = nov,
day = "2",
doi = "10.1016/j.celrep.2021.109915",
language = "English (US)",
volume = "37",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "5",
}