Dissecting primate early post-implantation development using long-term in vitro embryo culture

Yuyu Niu, Nianqin Sun, Chang Li, Ying Lei, Zhihao Huang, Jun Wu, Chenyang Si, Xi Dai, Chuanyu Liu, Jingkuan Wei, Longqi Liu, Su Feng, Yu Kang, Wei Si, Hong Wang, E. Zhang, Lu Zhao, Ziwei Li, Xi Luo, Guizhong CuiGuangdun Peng, Juan Carlos Izpisúa Belmonte, Weizhi Ji, Tao Tan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The transition from peri-implantation to gastrulation in mammals entails the specification and organization of the lineage progenitors into a body plan. Technical and ethical challenges have limited understanding of the cellular and molecular mechanisms that underlie this transition. We established a culture system that enabled the development of cynomolgus monkey embryos in vitro for up to 20 days. Cultured embryos underwent key primate developmental stages, including lineage segregation, bilaminar disc formation, amniotic and yolk sac cavitation, and primordial germ cell–like cell (PGCLC) differentiation. Single-cell RNA-sequencing analysis revealed development trajectories of primitive endoderm, trophectoderm, epiblast lineages, and PGCLCs. Analysis of single-cell chromatin accessibility identified transcription factors specifying each cell type. Our results reveal critical developmental events and complex molecular mechanisms underlying nonhuman primate embryogenesis in the early postimplantation period, with possible relevance to human development.

Original languageEnglish (US)
Article numbereaaw5754
JournalScience
Volume366
Issue number6467
DOIs
StatePublished - Nov 15 2019

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Primates
Embryonic Structures
Single-Cell Analysis
RNA Sequence Analysis
Germ Layers
Gastrulation
Yolk Sac
Endoderm
Macaca fascicularis
Human Development
Germ Cells
Chromatin
Embryonic Development
Cell Differentiation
Mammals
Transcription Factors
In Vitro Techniques

ASJC Scopus subject areas

  • General

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Dissecting primate early post-implantation development using long-term in vitro embryo culture. / Niu, Yuyu; Sun, Nianqin; Li, Chang; Lei, Ying; Huang, Zhihao; Wu, Jun; Si, Chenyang; Dai, Xi; Liu, Chuanyu; Wei, Jingkuan; Liu, Longqi; Feng, Su; Kang, Yu; Si, Wei; Wang, Hong; Zhang, E.; Zhao, Lu; Li, Ziwei; Luo, Xi; Cui, Guizhong; Peng, Guangdun; Belmonte, Juan Carlos Izpisúa; Ji, Weizhi; Tan, Tao.

In: Science, Vol. 366, No. 6467, eaaw5754, 15.11.2019.

Research output: Contribution to journalArticle

Niu, Y, Sun, N, Li, C, Lei, Y, Huang, Z, Wu, J, Si, C, Dai, X, Liu, C, Wei, J, Liu, L, Feng, S, Kang, Y, Si, W, Wang, H, Zhang, E, Zhao, L, Li, Z, Luo, X, Cui, G, Peng, G, Belmonte, JCI, Ji, W & Tan, T 2019, 'Dissecting primate early post-implantation development using long-term in vitro embryo culture', Science, vol. 366, no. 6467, eaaw5754. https://doi.org/10.1126/science.aaw5754
Niu, Yuyu ; Sun, Nianqin ; Li, Chang ; Lei, Ying ; Huang, Zhihao ; Wu, Jun ; Si, Chenyang ; Dai, Xi ; Liu, Chuanyu ; Wei, Jingkuan ; Liu, Longqi ; Feng, Su ; Kang, Yu ; Si, Wei ; Wang, Hong ; Zhang, E. ; Zhao, Lu ; Li, Ziwei ; Luo, Xi ; Cui, Guizhong ; Peng, Guangdun ; Belmonte, Juan Carlos Izpisúa ; Ji, Weizhi ; Tan, Tao. / Dissecting primate early post-implantation development using long-term in vitro embryo culture. In: Science. 2019 ; Vol. 366, No. 6467.
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abstract = "The transition from peri-implantation to gastrulation in mammals entails the specification and organization of the lineage progenitors into a body plan. Technical and ethical challenges have limited understanding of the cellular and molecular mechanisms that underlie this transition. We established a culture system that enabled the development of cynomolgus monkey embryos in vitro for up to 20 days. Cultured embryos underwent key primate developmental stages, including lineage segregation, bilaminar disc formation, amniotic and yolk sac cavitation, and primordial germ cell–like cell (PGCLC) differentiation. Single-cell RNA-sequencing analysis revealed development trajectories of primitive endoderm, trophectoderm, epiblast lineages, and PGCLCs. Analysis of single-cell chromatin accessibility identified transcription factors specifying each cell type. Our results reveal critical developmental events and complex molecular mechanisms underlying nonhuman primate embryogenesis in the early postimplantation period, with possible relevance to human development.",
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AU - Li, Chang

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AU - Huang, Zhihao

AU - Wu, Jun

AU - Si, Chenyang

AU - Dai, Xi

AU - Liu, Chuanyu

AU - Wei, Jingkuan

AU - Liu, Longqi

AU - Feng, Su

AU - Kang, Yu

AU - Si, Wei

AU - Wang, Hong

AU - Zhang, E.

AU - Zhao, Lu

AU - Li, Ziwei

AU - Luo, Xi

AU - Cui, Guizhong

AU - Peng, Guangdun

AU - Belmonte, Juan Carlos Izpisúa

AU - Ji, Weizhi

AU - Tan, Tao

PY - 2019/11/15

Y1 - 2019/11/15

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