Distinct cellular localization and regulation of endothelin-1 and endothelin-converting enzyme-1 expression in the bovine corpus luteum: Implications for luteolysis

Nitzan Levy, Miri Gordin, Roni Mamluk, Masashi Yanagisawa, Michael F. Smith, Jim H. Hampton, Rina Meidan

Research output: Contribution to journalArticle

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Abstract

Endothelin-1 (ET)-1 within the corpus luteum (CL) is rapidly up-regulated during natural or PGF-induced luteolysis; however, such an increase was not observed at early luteal stage, when the CL is refractory to PGF. The mature and active form of ET-1 is derived from the inactive intermediate peptide, big ET-1, by ET-converting enzyme (ECE)-1. This study therefore examined the developmental and cell-specific expression of ECE-1 in bovine CL. A significant, 4-fold, elevation in ECE-1 expression (mRNA and protein levels) occurred during the transition of the CL from early to midluteal phase. Analysis using in-situ hybridization and enriched luteal cell subpopulations showed that both steroidogenic and endothelial cells of the CL expressed high levels of ECE-1 mRNA; prepro ET-1 mRNA, on the other hand, was only expressed by resident endothelial cells. These data suggest that luteal parenchymal and endothelial cells may cooperate in the biosynthesis of mature bioactive ET-1. In the mature CL, ECE-1 mRNA increase occurred both in steroidogenic and endothelial cells and was accompanied by a significant rise in ET-1 peptide. However, in contrast to ECE-1, prepro ET-1 mRNA levels were similar in early and midluteal-phase CL. Low ECE-1 levels during the early luteal phase, restricting the production of active ET-1, may explain why the immature CL is able to withstand PGF-induced luteolysis.

Original languageEnglish (US)
Pages (from-to)5254-5260
Number of pages7
JournalEndocrinology
Volume142
Issue number12
DOIs
StatePublished - 2001

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Luteolysis
Corpus Luteum
Endothelin-1
Dinoprost
Enzymes
Endothelial Cells
Messenger RNA
Luteal Cells
Endothelin-Converting Enzymes
Peptides
Luteal Phase
In Situ Hybridization

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Distinct cellular localization and regulation of endothelin-1 and endothelin-converting enzyme-1 expression in the bovine corpus luteum : Implications for luteolysis. / Levy, Nitzan; Gordin, Miri; Mamluk, Roni; Yanagisawa, Masashi; Smith, Michael F.; Hampton, Jim H.; Meidan, Rina.

In: Endocrinology, Vol. 142, No. 12, 2001, p. 5254-5260.

Research output: Contribution to journalArticle

Levy, Nitzan ; Gordin, Miri ; Mamluk, Roni ; Yanagisawa, Masashi ; Smith, Michael F. ; Hampton, Jim H. ; Meidan, Rina. / Distinct cellular localization and regulation of endothelin-1 and endothelin-converting enzyme-1 expression in the bovine corpus luteum : Implications for luteolysis. In: Endocrinology. 2001 ; Vol. 142, No. 12. pp. 5254-5260.
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