Distribution of cardiac myosin isozymes in human conduction system. Immunohistochemical study using monoclonal antibodies

M. Kuro-o, H. Tsuchimochi, S. Ueda, F. Takaku, Y. Yazaki

Research output: Contribution to journalArticle

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Abstract

To determine the presence and distribution of cardiac myosin isozymes in the human conduction system, we performed an immunohistochemical study using monoclonal antibodies CMA19 and HMC14, which are specific for myosin heavy chains of human atrial type (α-type) and ventricular type (β-type), respectively. Serial frozen sections of human hearts were obtained from autopsy samples and examined by indirect immunofluorescence. α-Type was found in all myofibers of sinus node and atrio-ventricular node, and in 55.2 ± 10.2% (mean ± SD, n = 5) of the myofibers of ventricular conduction tissue, which consists of the bundle of His, bundle branches, and the Purkinje network. In contrast, β-type was found in all myofibers of the atrio-ventricular node and ventricular conduction tissue, whereas almost all myofibers of the sinus node were unlabeled by HMC14. Although the number of ventricular myofibers labeled by CMA19 was small, the labeled myofibers were more numerous in the subepicardial region than in the subendocardial region. These findings show that the gene coding for α-type is expressed predominantly in specialized myocardium compared with the adjacent ordinary working myocardium.

Original languageEnglish (US)
Pages (from-to)340-347
Number of pages8
JournalJournal of Clinical Investigation
Volume77
Issue number2
StatePublished - 1986

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Cardiac Myosins
Isoenzymes
Bundle of His
Atrioventricular Node
Sinoatrial Node
Monoclonal Antibodies
Myocardium
Myosin Heavy Chains
Frozen Sections
Indirect Fluorescent Antibody Technique
Autopsy
Genes

ASJC Scopus subject areas

  • Medicine(all)

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Distribution of cardiac myosin isozymes in human conduction system. Immunohistochemical study using monoclonal antibodies. / Kuro-o, M.; Tsuchimochi, H.; Ueda, S.; Takaku, F.; Yazaki, Y.

In: Journal of Clinical Investigation, Vol. 77, No. 2, 1986, p. 340-347.

Research output: Contribution to journalArticle

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abstract = "To determine the presence and distribution of cardiac myosin isozymes in the human conduction system, we performed an immunohistochemical study using monoclonal antibodies CMA19 and HMC14, which are specific for myosin heavy chains of human atrial type (α-type) and ventricular type (β-type), respectively. Serial frozen sections of human hearts were obtained from autopsy samples and examined by indirect immunofluorescence. α-Type was found in all myofibers of sinus node and atrio-ventricular node, and in 55.2 ± 10.2{\%} (mean ± SD, n = 5) of the myofibers of ventricular conduction tissue, which consists of the bundle of His, bundle branches, and the Purkinje network. In contrast, β-type was found in all myofibers of the atrio-ventricular node and ventricular conduction tissue, whereas almost all myofibers of the sinus node were unlabeled by HMC14. Although the number of ventricular myofibers labeled by CMA19 was small, the labeled myofibers were more numerous in the subepicardial region than in the subendocardial region. These findings show that the gene coding for α-type is expressed predominantly in specialized myocardium compared with the adjacent ordinary working myocardium.",
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AB - To determine the presence and distribution of cardiac myosin isozymes in the human conduction system, we performed an immunohistochemical study using monoclonal antibodies CMA19 and HMC14, which are specific for myosin heavy chains of human atrial type (α-type) and ventricular type (β-type), respectively. Serial frozen sections of human hearts were obtained from autopsy samples and examined by indirect immunofluorescence. α-Type was found in all myofibers of sinus node and atrio-ventricular node, and in 55.2 ± 10.2% (mean ± SD, n = 5) of the myofibers of ventricular conduction tissue, which consists of the bundle of His, bundle branches, and the Purkinje network. In contrast, β-type was found in all myofibers of the atrio-ventricular node and ventricular conduction tissue, whereas almost all myofibers of the sinus node were unlabeled by HMC14. Although the number of ventricular myofibers labeled by CMA19 was small, the labeled myofibers were more numerous in the subepicardial region than in the subendocardial region. These findings show that the gene coding for α-type is expressed predominantly in specialized myocardium compared with the adjacent ordinary working myocardium.

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