Diurnal Oscillations in Liver Mass and Cell Size Accompany Ribosome Assembly Cycles

Flore Sinturel, Alan Gerber, Daniel Mauvoisin, Jingkui Wang, David Gatfield, Jeremy J. Stubblefield, Carla B. Green, Frédéric Gachon, Ueli Schibler

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The liver plays a pivotal role in metabolism and xenobiotic detoxification, processes that must be particularly efficient when animals are active and feed. A major question is how the liver adapts to these diurnal changes in physiology. Here, we show that, in mice, liver mass, hepatocyte size, and protein levels follow a daily rhythm, whose amplitude depends on both feeding-fasting and light-dark cycles. Correlative evidence suggests that the daily oscillation in global protein accumulation depends on a similar fluctuation in ribosome number. Whereas rRNA genes are transcribed at similar rates throughout the day, some newly synthesized rRNAs are polyadenylated and degraded in the nucleus in a robustly diurnal fashion with a phase opposite to that of ribosomal protein synthesis. Based on studies with cultured fibroblasts, we propose that rRNAs not packaged into complete ribosomal subunits are polyadenylated by the poly(A) polymerase PAPD5 and degraded by the nuclear exosome.

Original languageEnglish (US)
Pages (from-to)651-663.e14
JournalCell
Volume169
Issue number4
DOIs
StatePublished - May 4 2017

Fingerprint

Ribosomes
Cell Size
Liver
Polynucleotide Adenylyltransferase
Exosomes
Ribosome Subunits
Detoxification
Ribosomal Proteins
Physiology
Photoperiod
Protein Biosynthesis
Xenobiotics
Fibroblasts
rRNA Genes
Metabolism
Hepatocytes
Fasting
Animals
Proteins
Genes

Keywords

  • cell size
  • circadian
  • diurnal
  • feeding-fasting rhythms
  • liver
  • mouse
  • ribosomal protein synthesis
  • rRNA degradation
  • rRNA polyadenylation
  • TRAMP complex

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Sinturel, F., Gerber, A., Mauvoisin, D., Wang, J., Gatfield, D., Stubblefield, J. J., ... Schibler, U. (2017). Diurnal Oscillations in Liver Mass and Cell Size Accompany Ribosome Assembly Cycles. Cell, 169(4), 651-663.e14. https://doi.org/10.1016/j.cell.2017.04.015

Diurnal Oscillations in Liver Mass and Cell Size Accompany Ribosome Assembly Cycles. / Sinturel, Flore; Gerber, Alan; Mauvoisin, Daniel; Wang, Jingkui; Gatfield, David; Stubblefield, Jeremy J.; Green, Carla B.; Gachon, Frédéric; Schibler, Ueli.

In: Cell, Vol. 169, No. 4, 04.05.2017, p. 651-663.e14.

Research output: Contribution to journalArticle

Sinturel, F, Gerber, A, Mauvoisin, D, Wang, J, Gatfield, D, Stubblefield, JJ, Green, CB, Gachon, F & Schibler, U 2017, 'Diurnal Oscillations in Liver Mass and Cell Size Accompany Ribosome Assembly Cycles', Cell, vol. 169, no. 4, pp. 651-663.e14. https://doi.org/10.1016/j.cell.2017.04.015
Sinturel F, Gerber A, Mauvoisin D, Wang J, Gatfield D, Stubblefield JJ et al. Diurnal Oscillations in Liver Mass and Cell Size Accompany Ribosome Assembly Cycles. Cell. 2017 May 4;169(4):651-663.e14. https://doi.org/10.1016/j.cell.2017.04.015
Sinturel, Flore ; Gerber, Alan ; Mauvoisin, Daniel ; Wang, Jingkui ; Gatfield, David ; Stubblefield, Jeremy J. ; Green, Carla B. ; Gachon, Frédéric ; Schibler, Ueli. / Diurnal Oscillations in Liver Mass and Cell Size Accompany Ribosome Assembly Cycles. In: Cell. 2017 ; Vol. 169, No. 4. pp. 651-663.e14.
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